血栓素A_2受体通过介导环氧酶2的合成增强类风湿关节炎滑膜细胞的增殖作用  

Thromboxane A_2 receptor induces proliferation of rheumatoid arthritis synovial cells by up-regulation of cyclooxygenase-2

作　　者：储永良[1,2] 黄清春[1,2] 黄闰月[1,2] 晏靖遥 陈秀敏[1,2] 徐侦雄[1,2] 

机构地区：[1]广东省中医院 [2]广州中医药大学第二附属医院风湿科,广东广州510006

出　　处：《中国病理生理杂志》2014年第6期1110-1113,1118,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金青年科学基金资助项目(No.81302799);广东省中医院2012年风湿科院内专项(No.E418)

摘　　要：目的:研究血栓素A2受体(thromboxane A2receptor,TXA2R)作为环氧酶2(cyclooxygenase-2,COX-2)下游产物对类风湿关节炎(rheumatoid arthritis,RA)滑膜细胞增殖力和COX-2表达的影响。方法:利用细胞增殖与毒性检测试剂盒(MTS)检测TXA2R拮抗剂SQ29548和激动剂U46619对RA关节滑膜细胞MH7A增殖力的影响作用,并用real-time PCR检测它们对COX-2 mRNA表达的影响;利用BrdU细胞增殖检测法观察MH7A细胞在转染COX-2小干扰RNA(small interfering RNA,siRNA)后细胞增殖受抑制的情况及额外施加U46619的可能影响。结果:SQ29548和U46619分别具有抑制和促进MH7A细胞增殖力与COX-2 mRNA表达的作用,且U46619可在一定程度上重建由COX-2 siRNA所抑制的MH7A细胞增殖力。结论:TXA2通过其受体TXA2R既可控制COX-2的表达,又可介导COX-2的细胞增殖效应,有可能作为RA治疗较为理想的新靶标。AIM：To examine the effects of thromboxane A 2 receptor （ TXA2 R） , the downstream product of cyclooxygenase-2 （COX-2）, on the proliferative ability and COX-2 expression in rheumatoid arthritis （RA） synovial cells. METHODS：The effects of TXA2 R antagonist SQ29548 and agonist U46619 on the proliferation of RA synovial cell line MH7A were detected by MTS cell proliferation assay , and their effects on COX-2 mRNA expression in MH7A cells were also examined by realtime PCR.In addition, the possible effect of U46619 on the proliferation of MH7A cells, when COX-2 was knocked down by siRNA , was determined by BrdU cell proliferation assay .RESULTS：SQ29548 inhibited the cell proliferation and the mRNA level of COX-2 while U46619 enhanced them.Moreover, U46619 reconstitute the proliferative ability of MH7A cells to some extent that inhibited by COX-2 siRNA.CONCLUSION： In RA synovial cells, TXA2R is able to control COX-2 expression, while it also mediates the effects of COX-2, suggesting that TXA2R might be an ideal candidate for RA treatment .

关 键 词：关节炎 类风湿 血栓素A2受体 环氧酶2 滑膜细胞 

分 类 号：R593.22[医药卫生—内科学]