构建仿生解剖外形的个体化人工骨的新方法  

A novel method to fabricate customized scaffolds with anatomical shape

作　　者：杜大江[1] 刘真[2] 邵林[1] 韩剑锋[1] 牛田多加志[3] 古川克子[3] 

机构地区：[1]哈尔滨医科大学附属第二医院骨科,黑龙江哈尔滨150081 [2]哈尔滨医科大学附属第二医院科研实验中心,黑龙江哈尔滨150081 [3]东京大学工学部再生医工学研究室

出　　处：《哈尔滨医科大学学报》2014年第3期195-197,201,共4页Journal of Harbin Medical University

基　　金：黑龙江省自然科学基金资助项目(LC201020);黑龙江省卫生厅科研课题(2010-094);哈尔滨市科技创新人才专项基金(2011RFLYSO31)

摘　　要：目的研究利用微球胶联法和快速成形技术制备具备解剖结构的个体化人工骨的可行性。方法首先制备三磷酸钙微球,以兔股骨的一段作为重建模型,通过CT图像重建三维数字模型。利用反求法建立模板数据模型,再用激光固化快速成形法制备出模板实体。将三磷酸钙微球填充入模板,藻酸胶联塑形,去除模板后对个体化人工骨进行形态学观察分析。结果成功制备出三磷酸钙微球,并根据CT图像借助激光固化快速成形技术成功制备出光硬化树脂模板。将微球填充入模板并用藻酸钠水凝胶交联塑形,成功获得与兔股骨解剖外形一致的个体化人工骨。结论微球胶联法结合激光快速成形技术可以成功制备具有解剖外形结构的个体化人工骨,该方法作为间接法制备个体化生物陶瓷支架的新技术可以应用于组织工程中。Objective To study the feasibility of fabricating customized artificial bone graft by cross-linking micro-beads and stereolithography technique. Methods The micro-beads of beta-TCP was fabricated first, and the 3D digital model of a segment of rabbit femur and the negative UV-cured resin mould were made by rapid prototyping. Then the mold was filled by the beads and cross-link the beads was filled by alginate hydrogel. Morphological study of the fabricated artificial bone graft was performed after removing the mould. Results The micro-beads of beta-TCP were successfully fabricated. The negative mold was produced successfully by stereolithography. After filling the beads into the mould and binding them together by cross-linked alginate hydrogel, A customized artificial bone graft with anatomical shape the same as the rabbit femur was achieved successfully. Conclusion Customized artificial bone graft could be produced by the novel method by cross-linking micro-beads and stereolithography technique and it could be useful for tissue engineering.

关 键 词：快速成形 三磷酸钙 解剖外形 组织工程 陶瓷支架 

分 类 号：R318.1[医药卫生—生物医学工程]