血培养病原菌的分布及耐药性分析  

Distribution and drug resistance of bacteria isolated from blood specimens

作　　者：郭秀臣[1] 郭微媛[2] 

机构地区：[1]哈尔滨医科大学附属第三医院血液科 [2]哈尔滨医科大学附属第二医院检验科,黑龙江哈尔滨150081

出　　处：《哈尔滨医科大学学报》2014年第3期230-233,共4页Journal of Harbin Medical University

摘　　要：目的了解血培养中病原菌的分布及耐药情况,以指导临床合理用药。方法采用BACTEFX型全自动血培养仪进行培养,MicroScan WalkAway 96SI全自动微生物鉴定仪进行鉴定和药敏试验。结果5 879例血培养标本中分离出950株病原菌,阳性率占16.2%。其中,革兰氏阳性菌占52.9%,革兰氏阴性菌占43.4%,真菌占3.7%。最常见的病原菌分别是凝固酶阴性葡萄球菌、大肠埃希菌、金黄色葡萄球菌。未发现耐万古霉素的革兰氏阳性球菌,革兰氏阴性杆菌对亚胺培南高度敏感。结论血培养病原菌种类复杂,耐药率高,临床医生应及时监测血培养中病原菌的变化及耐药趋势,采取合理的抗菌治疗,控制院内感染。Objective To investigate the distribution and drug resistance of pathogenic bacteria in blood culture and to conduct usage of antimicrobial agents in clinic. Methods The blood samples were cultivated in the BACTEFX system. Isolated colonies were identified and drug sensitivity was tested by Microscan WalkAway 96SI system. Results Strains of pathogenic bacteria were isolated from 5 879 blood specimens, the positive rate was 16. 2%. Gram-positive and Gram-negative bacilli isolated accounted for 52. 9% and 43.4% , respectively, and fungi for 3.7%. The most common pathogens were coagulase negative Staphylococcus, Escherichia coli, Staphylococcus aureus. No strains resistant to vaneomycin was found in Gram-positive bacteria. Gram-negative bacilli were highly susceptible to imipenem. Conclusion There are many kinds of pathogens identified from blood culture, and the drug resistance rate is high. Clinicians should monitor the change of pathogens and trends of drug resistance in time in order to give the patient correct antibiotic therapy and to control cross-infection in hospitals.

关 键 词：血培养 病原菌 耐药性 

分 类 号：R378[医药卫生—病原生物学]