尤溪金柑离体再生体系优化及试管苗保存  

Optimization of plantlet regeneration system and in vitro conservation of Fortunella crassifolia cv.Youxijingan

作　　者：金建涛[1] 赖钟雄[1] 刘生财[1] 林玉玲[1] 张梓浩[1] 

机构地区：[1]福建农林大学园艺植物生物工程研究所,福州350002

出　　处：《福建农林大学学报（自然科学版）》2014年第3期256-262,共7页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：福建省重大科技平台建设项目(2008N2001);国家支撑计划资助项目(2007BAD07B01)

摘　　要：以尤溪金柑试管苗为材料,采用双因素试验设计,筛选出适合尤溪金柑试管苗增殖和生根的培养基;采用L3(34)正交表设计,研究CaCl2、甘露醇和多效唑对尤溪金柑试管苗保存的影响.结果表明:芽增殖的最优培养基为:MS+1.2 mg·L-16-BA+0.05 mg·L-1NAA,增殖系数最高,达10.60;生根最优培养基为:MS+0.3 mg·L-1NAA+0.2 mg·L-1IBA,生根率最高达到了83.33%.保存12个月时处理C4(MS+0.44 g·L-1CaCl2+0 g·L-1甘露醇+1.0 mg·L-1多效唑)中尤溪金柑试管苗的存活率均为最高,达97.8%.In vitro plantlets were used as the materials for the studies of optimization of regeneration system, the media for micro-propagation, growth and roofing of tube plantlets were optimized in Fortunella crassifolia cv. Youxijingan. The influences of differentconcentrations of PP333, CaCl2 and manuitol on the conservation of in vitro plantlets were compared. The results showed that the bestproliferation medium was MS ＋ 1.2 mg·L-1 6-BA ＋0.05 mg·L-1 NAA, the proliferation coefficient reached 10.60; the best roo-fing medium was MS ＋0.3 mg·L-1NAA ＋0.2 mg·L-1 IBA, the root induction rate reached 83.33% ; the best proliferation medi-um was C4 （ MS ＋ 0.44 g·L-1 CaCl2 ＋ 0 g·L-1 manuitol ＋ 1.0 mg·L-1 PP333 ）, it was suitable for in vitro conservation of For-tunella crassifolia cv. Youxijingan plantlets, and the survival rate reached 97.8% without subculture for 12 months.

关 键 词：尤溪金柑 离体再生体系 优化 试管苗 离体保存 

分 类 号：S666[农业科学—果树学]