香蜂花的离体保存及试管苗抗氧化酶活性的变化  

In vitro conservation and the changes of antioxidase activity of Melissa officinalis plantlets

作　　者：姚凤琴[1,2] 赖钟雄[1] 

机构地区：[1]福建农林大学园艺植物生物工程研究所,福建福州350002 [2]三明市农业科学研究院,福建三明365509

出　　处：《福建农林大学学报（自然科学版）》2014年第3期263-268,共6页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：福建省亚热带果树及特种经济作物种质资源共享平台项目(2008N2001);国家科技支撑计划项目(2007BAD07B03)

摘　　要：以香蜂花试管苗为材料,研究不同浓度ABA和不同因素(白糖、琼脂、甘露醇、多效唑)对香蜂花试管苗离体保存的影响,并且测定试管苗在最佳培养基保存过程中抗氧化酶活性的变化.结果表明:适宜香蜂花试管苗离体保存的最佳培养基为MS+20 g·L-1白糖+9 g·L-1琼脂+10 g·L-1甘露醇+7 mg·L-1多效唑;同时发现最佳培养基与对照MS培养基中试管苗的抗氧化酶(SOD、CAT及POD)活性的变化趋势都是先上升后下降,但最佳培养基上的试管苗的酶活性要高于对照并且延迟了酶活性最高峰出现的时间.The effects of in vitro conservation on Melissa offtcinalis planflets under different concentrations of ABA and different factors （sugar, agarophyte, mannitol, paclobutrazol）, and the changes in activities of antioxidase of the planflets fi＇om different mediawere examined. The results showed that the best medium for in vitro conservation of Melissa officinalis plantlets was MS medium with20 g·L-1 sugar, 9 g·L-1 agarophyte, 10 g·L-1 mannitol and 7 mg·L-1 paclobutrazol. Additionally, trends of these antioxl-dase （SOD, CAT, and POD） activities in vitro conservation of Melissa officinalis plant.lets on both the best medium and the MS medium were accordant, rose at first and then declined. But the antioxidase activity of the plantlets kept on the best medium was higherthan that on the MS medium and delayed the time of the peak of enzyme activity.

关 键 词：香蜂花 离体保存 抗氧化酶活性 

分 类 号：S681.9[农业科学—观赏园艺]