大白猪MUC13基因多态性与生长、肉质性状的相关性  

The polymorphism of MUC13 gene of large white pig and its correlation with the traits of growth and meat quality

作　　者：刘亚轩 肖石军[2] 郭长明 陈福珍 林金玉 陈晓燕 沈金清 杨锋 林长光[1,3] 

机构地区：[1]福建光华百斯特生态农牧发展有限公司,福建尤溪365106 [2]江西农业大学动物生物技术国家重点实验室培育基地,江西南昌330045 [3]福建省农业科学院畜牧兽医研究所,福建福州350013

出　　处：《福建农林大学学报（自然科学版）》2014年第3期295-298,共4页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家生猪产业技术体系建设项目(CARS-36);福建省科技厅区域重大专项(2011N3022);福建省科技重大专项(2012NZ0003-3);福建省生猪产业技术体系项目(闽农科教[2009]200号)

摘　　要：选择对仔猪腹泻有显著影响的产肠毒素大肠杆菌(ETEC)F4ac的受体基因MUC13为研究对象,采用PCR-SNaPshot判型与常规育种技术相结合的方法,对544头大白猪核心育种群进行基因判定及基因型与生长、肉质性状相关性的研究.结果表明,大白猪MUC13基因型分布大小为:腹泻易感杂合基因型GA>腹泻抗性纯合基因型GG>腹泻易感纯合基因型AA,腹泻抗性有利基因G的等位基因频率为0.669,腹泻易感不利基因A的等位基因频率为0.331.3种基因型个体达100kg日龄及其估计育种值(EBV)、达100 kg背膘厚及其EBV的大小为:AA>GA>GG;眼肌面积、肌内脂肪含量和估计瘦肉率的大小为:GG>GA>AA,差异不显著(P>0.05).可见,在种猪选育上,逐代选择带有G等位基因的个体,建立抗性纯合GG基因型个体的种猪核心群,即可降低仔猪腹泻率,又不影响生长和肉质的性状.With 544 nucleus breeding large white pigs as material, PCR-SNaPshot and conventional breeding test was used to deter-mine the genotypes, and the correlations between genotypes of MUCl3 and the traits of growth and meat quality were analyzed. Theresult showed that the frequencies of 3 genotypes were GA 〉 GG 〉 A.A, G allele frequency was 0.669, A allele frequency was 0.331.The ages of 100 kg body weight and its estimated breeding value （EBV） of the 3 genotypes were AA 〉 GA 〉 GG, as well as the backfat thickness of 100 kg body weight and its EBV. Eye muscle area, intramuscular fat content, and estimated lean meat percentage ofthe 3 genotypes were GG 〉GA 〉AA , which were not significant （P 〉0.05）. Therefore, in the selection process of pig breeding, theindividuah with allele G should be selected generation-by-generation for reducing diarrhea rate of piglets, establishing a favorable gen-otype of MUCl3 pure line core group, which would not affect the growth rate and meat quality traits in the breeding large white pigs.

关 键 词：大白猪 MUC13基因 生长性状 肉质性状 

分 类 号：S813.3[农业科学—畜牧学]