芪桂痛风片对痛风性关节炎动物模型的镇痛研究  

Analgesic effects of Qigui Tongfeng Tablets on rats and mice with gouty arthritis

作　　者：邓奕[1,2,3] 张红[1,2,3] 曹亮[1,2,3] 丁岗[1,2,3] 王振中[1,2,3] 萧伟[1,2,3] 

机构地区：[1]江苏康缘药业股份有限公司,江苏连云港222001 [2]中药制药过程新技术国家重点实验室,江苏连云港222001 [3]江苏省企业院士工作站,江苏连云港222001

出　　处：《现代药物与临床》2014年第6期589-593,共5页Drugs & Clinic

基　　金：国家重大新药创制项目(2013ZX09402203)

摘　　要：目的观察芪桂痛风片对大鼠关节炎模型、小鼠热板和醋酸致小鼠扭体模型的影响。方法给予芪桂痛风片5 d后,观察芪桂痛风片对尿酸钠致大鼠关节炎模型痛阈改变的影响;采用小鼠热板模型,观察小鼠给予芪桂痛风片后舔足潜伏期的变化;小鼠ip 0.7%醋酸,观察给予芪桂痛风片后对小鼠扭体反应次数和扭体潜伏期的影响。结果大鼠关节炎模型造模1 h后,芪桂痛风片8.10 g/kg剂量组大鼠痛阈降低值和降低率与模型组比较差异具有显著性(P<0.01);造模3、5 h后,芪桂痛风片4.05、8.10 g/kg剂量组大鼠痛阈降低值和降低率较模型组低,且差异均具有显著性(P<0.05、0.01)。末次给药60、90、120 min后,芪桂痛风片各剂量(11.70、5.85、2.93 g/kg)组小鼠舔足潜伏期均比模型组延长。芪桂痛风片各剂量(11.70、5.85、2.93 g/kg)组小鼠扭体次数和扭体率均低于模型组,小鼠扭体潜伏期高于模型组。结论芪桂痛风片能够减轻尿酸钠注射引起的大鼠关节痛阈降低,并减轻小鼠热板和醋酸引起的疼痛,提示芪桂痛风片具有抗实验性痛风性关节炎作用。Objective To investigate the effects of the Qigui Tongfeng Tablets on monosodium urate crystal-induced arthritis in rats and mice, as well as to observe the analgesic effects in mice. Methods Pain threshold changes of rats with arthritis model induced by intra-articular injection of monosodium urate crystal suspension inside the ankle joint were observed after administrated Qigui Tongfeng Tablets for 3 d. The latency of licking foot was measured after being administrated Qigui Tongfeng Tablets in hot-plate model mice. The writhing times and writhing latency of mice with pain model induced by ip 0.7% acetic acid were observed after administrated Qigui Tongfeng Tablets. Results There was significant difference （P〈0.01） between the model group and 8.10 g/kg Qigui Tongfeng Tablets group in the value and rate of pain threshold changes after 1 h arthritis model being made, and there was also significant difference （P〈0.05, 0.01） between the model group and 4.05, 8.10 g/kg Qigui Tongfeng Tablets groups after 3,5 h. The latency of licking foot of mice in Qigui Tongfeng Tablets （11.70, 5.85, 2.93 g/kg） groups was longer than that of mice in the model group after 60, 90, and 120 min since last administration. The writhing times and writhing rats of mice in Qigui Tongfeng Tablets （11.70, 5.85, 2.93 g/kg） groups were lower than those of mice in the model group, while writhing latency opposite. Conclusion Qigui Tongfeng Tablets have the effects of reducing pain threshold decrease of arthritis model of rats induced by intra-articular injection, relieving the mice pain induced by hot-plate and acetic acid. It suggests that Qigui Tongfeng Tablets could exert the effect in the treatment of acute gouty arthritis.

关 键 词：芪桂痛风片 痛风性关节炎 关节炎试验 热板试验 醋酸扭体试验 

分 类 号：R285.5[医药卫生—中药学]