安徽省SMV株系的鉴定及其抗源筛选  

Identification of strains and screening of resistance resources to soybean mosaic virus in Anhui Province

作　　者：王大刚[1] 李华伟[2] 智海剑[2] 田震[2] 胡晨[1] 胡国玉[1] 黄志平[1] 张磊[1] 

机构地区：[1]安徽省农业科学院作物研究所/安徽省农作物品质改良重点实验室,安徽合肥230031 [2]南京农业大学大豆研究所/国家大豆改良中心/作物遗传与种质创新国家重点实验室,江苏南京210095

出　　处：《中国油料作物学报》2014年第3期374-379,共6页Chinese Journal of Oil Crop Sciences

基　　金：安徽省自然科学基金(1308085QC59);国家大豆产业技术体系建设项目(CARS-04);安徽省农业科学院院长青年创新基金(12B0204)

摘　　要：为明确安徽省大豆花叶病毒（soybean mosaic virus,SMV）株系的最新组成、分布及消长动态,以及筛选对流行株系的抗性种质,2009-2010年广泛采集安徽省各大豆主产区疑似SMV病样461份,采用生物学及血清学方法鉴定得到83个SMV分离物。用全国统一的10个鉴别寄主对这些分离物进行鉴定,共鉴定出14个SMV株系,如SC3~SC9、SC11、SC13~SC15、SC17、SC19和SC22,其中SC5、SC11、SC14、SC15、SC17、SC19和SC22是本研究在大豆主产区新发现的。进一步比较分析发现,SMV株系SC3和SC7仍为目前安徽省大豆产区的流行株系,分别占分离物总数的16.9%和25.3%;其次为新发现的强毒株系SC15,在安徽省也有较为广泛的分布,比率为12.1%。针对流行株系SC3和SC7的抗源筛选结果表明,来自参加安徽省区试的149份资源中有中作J8023、中黄45、L85-2308、中涡47号和濉科13等5份材料对SC3表现高抗,中黄45、宿03-4-1-4和鲁06-7等9份材料对SC7表现高抗;参加我国南方区试的95份种质资源中有13份对强毒株系SC15表现抗病,占鉴定品种的13.7%。本研究明确了安徽省大豆产区的流行株系及变化趋势,筛选出针对SMV流行株系的抗源,为培育抗病品种和抗病品种布局提供了依据。In order to clarify the local constitution, distribution, and population dynamics of soybean mosaic virus （SMV） in Anhui Province and to screen resistant accessions to epidemic SMV strains, 461 potential SMV samples of soybean leaves have been collected from main soybean regions in Anhui Province during 2009 - 2010. Of those, 83 SMV isolates were identified through expanding propagation, single lesion isolation purification, serological determination. They were grouped into the 14 SMV strains including SC3 -SC9, SCll, SC13 -SC15, SC17, SC19 and SC22, based on their response to 10 unified national soybean differentials. Of the 14 SMV strains ,7 strains were found for the first time in this study, encompassing SC5, SCII, SCI4, SC15, SC17, SC19 and SC22. The comparison between present and previous identification results of SMV strains showed that SC3 and SC7 strains were still predominant in Anhui Province with 16.9% and 25.3% of the total number of isolates, and SC15 was lower with 12.1%. In terms of soybean accessions resistant to popular strains of SC3 and SC7, 5 of 149 accessions, Zhongzuo J8023, Zhonghuang 45, L85 - 2308, Zhongguo 47 and Suike 13 showed high resistance to SC3 strain and 9 accessions to SC7, including Zhonghuang 45, Su03 - 4 - 1 - 4 and Lu06 - 7 etc. 13 accessions displayed resistant to SC15, account for 13.7% of the 95 tested soybean accessions. In this paper, we clarified the SMV predominant strains and their variation trend in the main soybean producing regions of Anhui Province, providing the basis for the breeding and layout of resistant soybean accessions to the disease.

关 键 词：大豆 大豆花叶病毒 株系鉴定 抗源筛选 

分 类 号：S435.651[农业科学—农业昆虫与害虫防治]