活性三七饮片指纹图谱研究及单体皂苷含量测定  

Study fingerprint chromatograms and determine monomer Saponins content of active Panax notoginseng medicine

作　　者：李琳[1,2] 王承潇[1,2] 崔秀明[1,2] 赵爱 

机构地区：[1]昆明理工大学生命科学与技术学院,云南昆明650500 [2]中药质量与资源开发滇澳联合实验室,云南昆明650500 [3]云南同根三七产业有限公司,云南文山663000

出　　处：《云南大学学报（自然科学版）》2014年第4期551-556,共6页Journal of Yunnan University(Natural Sciences Edition)

基　　金：国家自然科学基金-云南联合基金(U1032604)

摘　　要：HPLC方法具有良好的精密度、重复性和稳定性,能够分离活性三七饮片和传统干燥三七药材的各类组分,并可对其中三七皂苷R1、人参皂苷Rg1、Re、Rb1和Rd进行定量分析.研究采用HPLC法测定活性三七饮片与传统干燥三七药材的指纹图谱及主要单体皂苷含量,在此基础上,建立活性三七药材HPLC指纹图谱,并展开活性三七饮片与传统三七药材的指纹图谱差异性研究.结果表明,活性三七饮片的指纹图谱具有较好的专属性,适用于活性三七饮片的质量控制.The HPLC analysis was suitable for separating all kinds of components of active Panax notoginseng medicine and traditional drying Panax notoginseng medicine due to its sound precision, reproducibility and stability, the components can be separated well and the Rd can be determined. In this paper, the main monomer notoginsenosides R1 and ginsenosides Rg1, Re, Rb1 and saponins content and fingerprint chromatograms of active Panax notoginseng medicine and traditional drying Panax notoginseng medicine were determined by HPLC;and the HPLC fingerprint chromatogram of active Panax notoginsen medicine was established. It indicated that there was no difference between active Panax notoginseng and Panax notoginseng fingerprint. The fingerprint chromatogram established in this study was suitable for quality control of active Panax notoginsen products. The results showed that the fingerprint chromatogram of active Panax notoginsen medicine had good specificity, was suitable for quality control of active Panax notoginsen medicine.

关 键 词：活性三七 指纹图谱 质量标准 含量测定 

分 类 号：Q949.763.2[生物学—植物学] O629.13[理学—有机化学]