益肾调气法对脑出血后抑郁大鼠脑内BDNF表达的影响  

Influence of therapy of reinforcing kidney and regulating qi on BDNF expression in rats with post-encephalorrhagia depression

作　　者：罗斌[1] 唐启盛[1] 李侠[1] 徐向青[2] 

机构地区：[1]北京中医药大学第三附属医院,北京100029 [2]山东中医药大学附属医院

出　　处：《北京中医药大学学报》2014年第6期393-396,I0003,共5页Journal of Beijing University of Traditional Chinese Medicine

基　　金：北京中医药大学自主课题资助(No.2013-JYBZZ-JS-101)

摘　　要：目的观察脑出血后抑郁模型大鼠脑内脑源性神经营养因子(BDNF)的表达,研究益肾调气法中药颐脑解郁方治疗脑出血后抑郁的机理。方法以旷场法(open-field)进行行为学评分,选择得分相近的32只大鼠,随机分为正常组、模型组、中药颐脑解郁方组和百忧解组共4组,每组8只。采用免疫组织化学方法观察各组大鼠脑内BDNF的表达变化。结果模型组海马CA1、CA3区BDNF阳性细胞面密度、光密度,CA2区光密度较正常组显著减少(P<0.01),百忧解组CA1区BDNF阳性细胞面密度和CA3区面密度、光密度较模型组显著增加(P<0.01);中药组CA3区BDNF阳性细胞面密度、光密度较模型组增加(P<0.01)。与正常组相比,模型组扣带皮质面密度、光密度及下丘脑面密度显著降低(P<0.01);中药组扣带皮质光密度及下丘脑阳性细胞面密度较模型组显著增加(P<0.01)。结论益肾调气法中药颐脑解郁方能通过增加脑出血后抑郁模型大鼠BDNF的表达,促进神经元的修复与再生,改善抑郁症状。Objective To observe the expression of brain-derived neurotrophic factor （BDNF） in rats with post-encephalorrhagia depression, and study the mechanism of therapy of reinforcing kidney and regulating qi （Yinao Jieyu Fang） in treatment of post-encephalorrhagia depression. Methods The rats （ n = 32） with the similar scores of open-field test were chosen and randomly divided into normal group, model group, Chinese medicial group （treated with Yinao Jieyu Fang） and Western medicial group （treated with Baiyoujie, each n = 8 ）. The changes of BDNF expression were observed by using immunohistochemistry technique in normal group, model group, Chinese medicial group and Western medicial group. Results In model group, the areal density （AD）, optical density （OD） in hippocampus CA1 and CA3 zones and OD in CA2 zone of BDNF positive cells decreased significantly compared with normal group （P 〈 0.05 ） , and AD of BDNF positive cells in CA1 zone, and AD and OD in CA3 zone increased significantly in Western medicinal group compared with model group （ P 〈 0. 05 ）. Compared with normal group, AD and OD in cingulum cortex and AD in hypothalamus decreased significantly in model group （ P 〈 0. 05 ）. In Chinese medicial group, AD and OD of BDNF positive cells increased in CA3 zone compared with model group （ P 〈 0. 05 ）, OD increased significantly in cingulum cortex compared with model group, and AD increased in hypothalamus compared with model group （ P 〈 0. 05）. Conclusion Yinao Jieyu Fang can promote the repair and regeneration of neurons and relivedepression symptoms through increasing BDNF expression in rats with post-encephalorrhagia depression.

关 键 词：益肾调气法 脑出血后抑郁 脑源性神经营养因子 海马 扣带皮质 下丘脑 大鼠 

分 类 号：R285.5[医药卫生—中药学]