预适应对肢体缺血再灌注后血浆和肺组织NO/ET-1的影响  

Effect of ischemic preconditioning on the NO/ET-1 in the plasma and lung tissue following ischemia reperfusion in the hind limbs of rats

作　　者：高洪波[1] 郭文斌[2] 张连元[3] 

机构地区：[1]包头医学院,014040 [2]包头职业技术学院,014040 [3]河北联合大学

出　　处：《内蒙古中医药》2014年第15期113-114,共2页Inner Mongolia Journal of Traditional Chinese Medicine

基　　金：河北省教育厅课题(2001135)

摘　　要：目的:观察肢体缺血预适应对大鼠肢体缺血再灌注后血浆和肺组织NO/ET-1比值的影响。方法:将雄性Wistar大鼠随机分为对照组(C),肢体缺血再灌注组(LIR),缺血预适应组(IPC),每组8只。分别测定血浆及肺组织一氧化氮(NO)、内皮素(ET-1)含量,计算NO/ET-1比值。结果:LIR组血浆及肺组织的NO/ET-1比值明显减小;与LIR组比较,IPC组血浆及肺组织的NO/ET-1比值明显增大,差异均具有显著性。结论:缺血预适应可以调节肢体缺血再灌注后NO/ET-1比值失平衡而使组织损伤减轻。Abjective ：To study the effect of ischemic preconditioning on the NO/ET-1 in the plasma and lung tissue following ischemia reperfusion in the hind limbs of rats. Methods ：Wistar rats were randomly divided into three groups：control group ,limbs ischemia reperfusion（LIR） group,ischemia preconditioning （IPC）group. At the end of the experiment, The values of NO ,ET-1 in the plasma and lung tissue were measured. the ratio of NOfET-1 was calculated.the results were analysed quantitatively by relative computer analytical system. Results ： After the rats＇ hind limbs suffered ischemia-repcrfusion, the ratio of NO/ET-1 in the plasma and lung tissue were decreased significantly in the LIR group, Compared with LIR group, the ratio of NO/ET-lin the plasma and hmg tissue were increased signilicantly in the IPC group. Conclusion ：The IPC can attenuate lung injury following IR in the hind limbs of rats,which may corrdated with adjusting the ratio of NO/ET-1.

关 键 词：缺血预适应 再灌注损伤 一氧化氮 内皮素 

分 类 号：R33[医药卫生—人体生理学]