保肺定喘汤对COPD全身炎症反应及脂质代谢影响的实验研究  

Protective effects of Chinese herbal medicine Baofeidingchuan decoction on systemic inflammation and lipid metabolism in COPD rats

作　　者：徐志波[1] 王璐[1] 陈斌[2] 何薇[1] 胡敬献 刘贤忠[1] 陈芳[2] 

机构地区：[1]浙江中医药大学第一临床医学院,杭州310053 [2]浙江省中医院肺功能室

出　　处：《浙江医学》2014年第11期921-923,927,共4页Zhejiang Medical Journal

基　　金：国家自然基金项目(2012R10063);浙江省中医药管理局项目(2011ZQ009)

摘　　要：目的探讨保肺定喘汤对慢性阻塞性肺疾病(COPD)全身炎症反应及脂质代谢的影响。方法将40只SD大鼠随机分为4组:正常组10只、吸烟组30只(其中保肺定喘汤预防组10只、保肺定喘汤治疗组10只、COPD组10只)。除正常组外,其余3组大鼠在自制熏烟箱内被动吸烟24周,并于第30天和第45天于气道内滴入脂多糖。预防组开始吸烟时每天即予2ml保肺定喘汤灌胃,而治疗组在COPD出现后开始以同等体积保肺定喘汤灌胃治疗4周。实验结束后处死大鼠,检测血清瘦素、TNF-α、脂联素、甘油三酯等指标,肺组织标本行HE病理染色检查。结果 COPD组瘦素、脂联素、甘油三酯等均比保肺定喘汤预防组及治疗组减少,均有统计学差异(均P<0.05);而TNF-α则显著高于保肺定喘汤预防组及治疗组(均P<0.01);瘦素与TNF-α脂联素、甘油三酯呈负相关(r=-0.685、-0.652、-0.633,均P<0.05)。结论保肺定喘汤能够改善COPD全身慢性炎症反应及脂质代谢。Objective To investigate the effects of Baofeidingchuan decoction on systemic inflammation and lipid metabolism in rats with chronic obstructive pulmonary disease （COPD）.Methods Rat COPD model was induced by passive smoking for 24 weeks and dropping lipopolysaccharides into airway at d 30 and d 45.Rats in prevention group （n=10） were given 2ml Baofeidingchuan decoction by gavage before COPD induced and rats in treatment group （n=10） were given same volume of Baofeidingchuan decoction after COPD induced for 4 weeks.Rats in normal control （n=10） and in COPD model groups （n=10） were given 2ml normal saline by gavage for 4 weeks.At the end of experiments all rats were sacrificed,serum levels of leptin,adiponectin,triglycerides were measured and pathological examination of lung tissue was performed.Results Serum levels of leptin,adiponectin,triglycerides in COPD group were lower than those in prevention and treatment groups （all P＜0.05）,while serum TNF-α level in COPD group were significantly higher than that in prevention and treatment groups.Serum leptin levels were negatively correlated with TNF-α,adiponectin and triglycerides levels （r=-0.685,-0.652 and-0.633,allP＜0.05）.Conclusion Baofeidingchuan decoction can improve chronic systemic inflammation and lipid metabolism in rats with chronic obstructive pulmonary disease.

关 键 词：慢性阻塞性肺疾病 瘦素 脂联素 全身炎症反应 脂质代谢 

分 类 号：R285.5[医药卫生—中药学]