NDRG2截短体真核表达载体的构建  

Construction of eukaryotic expression vector expressing truncated NDRG2

作　　者：李惠晨 马勇政 王泽宇[2] 胡伟[2] 张健[1] 

机构地区：[1]第四军医大学基础部生物化学与分子生物学教研室,西安710032 [2]第四军医大学学员一旅

出　　处：《山东医药》2014年第23期9-12,共4页Shandong Medical Journal

基　　金：国家自然科学基金项目资助(81372390)

摘　　要：目的：构建稳定表达N-MYC下游调节基因2（NDRG2）截短体的真核表达载体。方法分别用PCR方法扩增NDRG2的截短体NDRG21～178aa和NDRG21～257aa ，以BamHⅠ和EcoRⅠ酶切后，以SolutionⅠ连接酶反应体系将NDRG2截短体片段装载入pCDNA4．0表达载体中，并通过蛋白免疫印迹（Western blotting）验证载体的表达。结果 NDRG2截短体在pCDNA4．0表达载体中正确表达。结论成功构建了NDRG2截短体的真核表达载体。Objective To construct pCNDA4.0-NDRG2 vectors that correctly express the truncated N-MYC down-stream-regulated gene 2 （NDRG2）.Methods The truncated NDRG21-178aa and NDRG21-257aa of NDRG2 was amplified by PCR, and were digested by BamHⅠand EcoRⅠ, then were cloned into the expression vector pCNDA 4.0 by SolutionⅠlig-ase reaction system.The protein expression of vectors was detected by Western blotting .Results The truncated NDRG2 was correctly expressed in the pCDNA 4.0 expression vector .Conclusion The eukaryotic expression vector of truncated NDRG2 is successfully constructed .

关 键 词：N-MYC下游调节基因2 截短突变体 pCNDA40载体 N-MYC downstream-regulated GENE 2 

分 类 号：Q782[生物学—分子生物学]