一株裂解性低温假单胞菌噬菌体的分离及特性研究  

Isolation and Characterization of a Lytic Cold-active Bacteriophage of Pseudomonas fluorescens

作　　者：孙策[1] 赵龙[1] 魏云林[1] 林连兵[1] 张琦[1] 季秀玲[1] 

机构地区：[1]昆明理工大学生命科学与技术学院,中国云南昆明650500

出　　处：《生命科学研究》2014年第3期222-226,共5页Life Science Research

基　　金：国家自然科学基金资助项目(31160121);云南省教育厅基金资助项目(2013Z138);中国科学院微生物研究所微生物资源前期开发国家重点实验室开放课题(SKLMR-20110606);云南省中青年科学与技术带头人后备人才项目(2009CI027)

摘　　要：从明永冰川低温土壤中分离、纯化获得一株低温假单胞菌噬菌体PFV1,并对其生物学特性（噬菌体基因组限制性酶切片段长度多态性、衣壳蛋白组分分析及生理特征）进行了初步研究。基于16S rRNA基因测序分析结果,将宿主菌初步鉴定为荧光假单胞菌菌株。噬菌体PFV1为球形,直径约50 nm。PFV1在4℃时具有侵染活性,4~25℃范围内均可形成噬菌。对氯仿具有一定的敏感性,具有热不稳定性,基因组为双链DNA,大小约38 kb。A lytic cold-active bacteriophage, designated as PFV1, was isolated.from soil sample of Mingyong glacier.Restriction fragment length polymorphism analysis of genomic DNA, constituent proteins and physiological analysis of PFVI were further carried out to characterize the phage. Based on the 16S rRNA gene sequence, the host strain was identified as a member of Pseudomonas fluorescens. The morphology of the isolated phage was observed by electron microscope. The bacteriophage is spherical, about 50 nm in diameter. Physiological analysis results showed that PFV1 had infection activity at 4 ℃, and plaques were formed between 4 and 25 ℃. It was sensitive to chloroform, and showed thermolability. Furthermore, the genome of PFV1 consists of double-stranded DNA and is approximately 38 kb.

关 键 词：荧光假单胞菌 低温噬菌体 分离 特性 

分 类 号：Q93[生物学—微生物学]