麦氏安瘿蜂体内侵染Wolbachia的WO噬菌体的检测及其orf7基因序列分析  

Sequencing and Phylogenetic Analysis of the orf 7 Gene of WO Phage in an Oak Gall Wasp, Andricus mairei(Hymenoptera: Cynipidae)

作　　者：苏成元[1] 杨筱慧[2] 朱道弘[1] 

机构地区：[1]中南林业科技大学昆虫行为与进化生态学实验室,中国湖南长沙410004 [2]湖南师范大学生命科学学院,中国湖南长沙410081

出　　处：《生命科学研究》2014年第3期227-231,共5页Life Science Research

基　　金：国家自然科学基金资助项目(30872036)

摘　　要：Wolbachia为节肢动物等的细胞质共生细菌,能对宿主的繁殖模式进行调控,包括诱导胞质不亲和、孤雌生殖、雌性化及雄性致死。WO噬菌体是一类侵染节肢动物体内Wolbachia的细菌病毒。为检测麦氏安瘿蜂Wolbachia是否被WO噬菌体侵染,本研究使用WO噬菌体的orf 7基因引物,对信阳、六安及长沙3种群的WO噬菌体进行了PCR检测。结果显示,3种群的雌、雄虫体内的Wolbachia均有WO噬菌体的侵染,感染率为100%。获得的3种群麦氏安瘿蜂Wolbachia的WO噬菌体orf 7基因序列均为371 bp,序列完全一致。其序列与粉斑螟Ephestia cautella的wECau B3-1和wCauA5-1株系、反颚茧蜂Asobara tabida的wAtabA3-3株系的一致性最高,在NJ和ML系统树中,均聚合在同一分支,属于WO噬菌体的第Ⅲ大群。Wolbachia species are members of the obligate intracellular Rickettsiales and parasite commonly in arthropods. Studies have showed that Wolbachia play an important role in reproduction of their hosts, including cytoplasmic incompatibility, feminization, parthenogenesis and male killing. WO phage is one of the bacterial viruses which infect Wolbachia. To test whether Wolbachia in A ndricus mairei Kieffer are infected by WO phage, DNA samples extracted from three widely separated geographic populations （Xinyang, Liuan, and Changsha） of A. mairei were run with primer of orf 7 gene of the WO by PCR. Results showed that WO phage infection rates were unusually high in the three tested populations： 100% for the wasps from all populations. The sequencing results showed that the orf 7 gene from all the three populations was 371 bp in length. Comparison of the orf 7 gene sequences of wAmaiA1-1 with wECau B3-1, wCauA5-1 and wAta- bA3-3 revealed a 99 % identity. It is suggested that phylogenic relationship of these phage WO strains is closely related and belongs to the Ⅲ group.

关 键 词：麦氏安瘿蜂 WOLBACHIA WO噬菌体 ORF 7基因 系统发育关系 

分 类 号：Q968[生物学—昆虫学]