艾灸足三里穴与关元穴对训练小鼠免疫功能的影响  

Effect of applying moxibustion at Zusanli and Guanyuan on training rat immunologic function

作　　者：容贤冰[1] 邓武装[2] 蒋晓明[3] 叶素英[4] 华岩[3] 

机构地区：[1]桂林电子科技大学体育部,广西桂林541004 [2]周口师范学院体育学院,河南周口466000 [3]钦州学院体育学院,广西钦州535000 [4]桂林航天工业学院体育部,广西桂林541004

出　　处：《重庆医学》2014年第17期2161-2163,共3页Chongqing medicine

基　　金：广西教育厅科学研究项目(2011 06XLS31);钦州学院科研项目(2012XJKY-15B)

摘　　要：目的探讨艾灸足三里、关元穴对训练小鼠免疫功能的影响。方法将30只雄性小鼠分为安静对照组(n=10)、运动训练组(n=10)、运动+艾灸组(n=10),后两组小鼠进行6周递增负荷训练,运动+艾灸组小鼠在每次训练后1h艾灸足三里、关元两个穴位,末次训练为负重8%的力竭游泳训练。测定各组小鼠血T淋巴细胞亚群、自然杀伤细胞(NK)、自然杀伤T(NKT)细胞及脾脏、胸腺抗氧化酶的活性变化情况。结果运动训练组与安静对照组相比,CD3+、CD4+/CD8+、NK、NKT、脾脏指数(SI)、胸腺指数(TI)降低(P<0.05),脾脏、胸腺组织中谷胱甘肽过氧化物酶(GSH-PX)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)降低(P<0.05),丙二醛(MDA)升高(P<0.05);运动+艾灸组与安静对照组相比,CD3+、CD4+/CD8+、NK、NKT降低(P<0.05),脾脏组织中MDA升高(P<0.05),胸腺组织中SOD升高(P<0.05),CAT降低(P<0.05);运动+艾灸组与运动训练组相比,CD3+、CD4+/CD8+、NK、NKT、SI、TI升高(P<0.05),脾脏、胸腺组织MDA降低(P<0.05),GSH-PX、SOD、CAT升高(P<0.05)。结论艾灸足三里穴、关元穴可提高训练小鼠外周血CD3+、NK、NKT细胞数量,预防CD4+/CD8+比值失调;同时抑制由于训练造成的TI、SI的变化趋势,增强胸腺、脾脏组织抗氧化酶的活性。提示艾灸足三里、关元穴可改善训练引起的免疫功能下降,提高机体免疫功能。Objective To study the effect of applying moxibustion at Zusanli and Guanyuan on training immunologic function. Methods 30 male rats were randomly divided into the sedentary control group （n=10）,training group （n=10）,training plus mox-ibustion group （n=10）.the six-week progressively increasing load training was carried out in the training group and the training plus moxibustion group,the rats in the training plus moxibustion group were given the 1 h moxibustion at the points of Zusanli and Guanyuan after each training and the exhaustive swimming with 8% of bear load was done in the last training.Then rats were killed after exhaustion for detecting the partial immune indexes in peripheral blood,thymus and spleen,and the activities of antioxidant en-zymes.Results Compared with sedentary control group,the ratio of peripheral blood CD3^＋,CD4^＋/CD8^＋,NK,NKT,index of spleen and thymus in the training group were significantly decreased（P〈0.05）,the content of MDA in spleen and thymus tissues were significantly increased （P〈0.05）,GSH-PX,SOD,CAT activities were decreased significantly （P〈0.05）;when compared with training group,peripheral blood CD3^＋,CD4^＋/CD8^＋,NK,NKT,the index of spleen and thymus in the training plus moxibus-tion group were significantly increased（P〈0.05）;the index of spleen and thymus is significantly increased（P〈0.05）,the content of MDA in spleen and thymus tissues were obviously decreased,GSH-PX,SOD,CAT were greatly increased（P〈0.05）.Conclusion Applying moxibustion at Zusanli and Guanyuan can increase the content of peripheral blood CD3＋,NK,NKT,prevent the ratio of CD4^＋/CD8^＋ from being imbalance,simultaneously inhibit the trends of changing in the index of thymus and spleen by the result of training and enhance the activity of antioxidant enzymes in thymus and spleen tissues,which implies that the moxibustion at Zusanli and Guanyuan can improve the immue function decline induced by the training and enhance the immune capacity of organ

关 键 词：针灸疗法 T淋巴细胞 自然杀伤细胞 胸腺 脾脏 

分 类 号：R245[医药卫生—针灸推拿学]