帕米尔地区野兔头骨标本制作方法  

Methods of preparing the specimens of skull bones from the hares in the Pamir region

作　　者：单文娟[1] 吴志强[1] 张琬琦 郑晓荣[1] 

机构地区：[1]新疆大学生命科学与技术学院新疆生物资源基因工程重点实验室,乌鲁木齐830046

出　　处：《黑龙江畜牧兽医》2014年第7期178-180,共3页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家自然科学基金项目(31301006);新疆大学本科生科研实践训练项目(XJU-SRT-13045)

摘　　要：为了筛选出制作帕米尔地区野兔头骨标本的最佳方法,试验采用虫蚀法、氧化法和碱腐蚀法分别制作帕米尔野兔头骨标本。结果表明:经高压灭菌锅在110℃、0.05 mPa条件下蒸煮20 min初步去肉后,以1.8%氢氧化钠(NaOH)浸泡14 h去肉、去脂,3.0%过氧化氢(H2O2)浸泡3 h漂白的碱腐蚀法为最佳方法。采用此方法制作标本省时、省力,头骨标本较为完整、美观。To select the best method for preparing specimens of skull bone from the hares in the Pamir region of Xinjiang, the worm - eaten method, oxidation method and alkaline etching method were used for preparing specimens of skull bone from the hares in the Pamir region, respectively. The results showed that the alkaline etching was the best method to prepare the specimens of skull bone of the hares. The alkaline etching method was processed in the following ways： after skull bones were boiled in autoclave sterilizer under the coudition of 110 ℃ with 0.05 mPa for initially removing meat, the skull bones were soaked in 1.8% sodium hydroxide （NaOH） for 14 h to remove the remained meat and lipids, and then in 3% hydrogen peroxide （ H202 ） for 3 h to bleach. The results indicate that this method is time - saving and efficient, and the prepared specimens of skull bone are more complete and beautiful.

关 键 词：头骨标本制作 碱腐蚀法 氧化法 虫蚀法 野兔 

分 类 号：S829.1[农业科学—畜牧学]