细梗香草总皂苷壳聚糖絮凝纯化工艺研究  

Flocculation and Purification of the Total Saponins of Lysimachia capillipes Hemsl.with Chitosan

作　　者：高帅[1,2] 李凌军[1] 栾明[1,2] 徐婵娟[2] 李锡敏 于秀颖[2] 张琳[2] 

机构地区：[1]山东中医药大学药学院,济南250014 [2]浙江大学生物医学工程与仪器科学学院,杭州310027 [3]常熟求是科技有限公司,江苏常熟215500

出　　处：《中国药学杂志》2014年第13期1113-1117,共5页Chinese Pharmaceutical Journal

基　　金：新药创制国家科技重大专项资助(2012ZX09102201-10)

摘　　要：目的研究细梗香草总皂苷壳聚糖絮凝纯化工艺,为继续纯化三萜皂苷B(LC-B)和三萜皂苷C(LC-C)奠定基础。方法用可见分光光度法、高效液相色谱-蒸发光散射检测法(HPLC-ELSD),分别测定总皂苷中间体水溶液絮凝率、总皂苷损失率,以絮凝率和总皂苷损失率为指标,用正交实验优选壳聚糖絮凝纯化最佳工艺条件。结果最佳工艺条件为总皂苷质量浓度为0.6 mg·mL-1,每100 mL总皂苷水溶液加入壳聚糖溶液30 mL,絮凝温度60℃,絮凝时间4 h。验证结果表明,总皂苷中间体水溶液澄清率为47.81%,总皂苷损失率为9.53%。结论与传统除鞣质工艺相比,壳聚糖絮凝纯化工艺能有效保留有效成分,工艺稳定性好,适合工业化生产。OBJECTIVE To study the flocculation process of the total saponins intermediate solution of Lysimachia capillipes Hemsl. with chitosan and lay the foundation for separation of the total saponins monomer components by medium pressure column. METHODS Visible spectrophotometry and HPLC-ELSD were used to respectively determine the flocculation rate and total saponins loss rate as two main parameters to estimate the effects of influencing factors on flocculation. The optimum conditions of chitosan floccu- lation were determined by orthogonal test. RESULTS The optimum conditions were as follows： aqueous solution concentration of 0. 6 mg ~ mL- 1, chitosan solution volume of 30 mL per 100 mL intermediate solution, flocculation temperature of 60 ~C, and flocculation time of 4 h. Under these conditions, a flocculation rate of 47.81% and a total saponins loss rate of 9. 53% were achieved. CONCLU- SION Compared with traditional tannin removing process, chitosan flocculation purification process can effectively retain the active ingredients in the solution and has good process stability, which is suitable for industrial production.

关 键 词：细梗香草 总皂苷 三萜皂苷B 三萜皂苷C 壳聚糖 高效液相色谱-蒸发光散射检测法 

分 类 号：R284[医药卫生—中药学]