高效液相色谱荧光检测法检测鸡组织中氨苄西林残留量  

Determination of Residual Amount of Ampicillin in Chicken Tissues by HPLC with Fluorescence Detection

作　　者：孙礼瑞 马晓萌[3] 谢恺舟[1,2] 张小杰[1,2] 王剑锋[1,2] 刘建宇[1,2] 崔璐璐[1,2] 张跟喜[1,2] 戴国俊[1,2] 王金玉[1,2] 

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]江苏省动物遗传繁育与分子设计重点实验室,江苏扬州225009 [3]青岛农业大学动物科技学院,山东青岛266000

出　　处：《安徽农业科学》2014年第20期6615-6617,6620,共4页Journal of Anhui Agricultural Sciences

基　　金：国家肉鸡产业技术体系专项(CARS-42-G23);江苏高校优势学科建设工程资助项目;江苏省普通高校研究生科研创新计划(CXLX12_0935);扬州大学"新世纪人才工程"资助项目

摘　　要：[目的]为氨苄西林在鸡组织中的残留检测标准的制定提供科学依据。[方法]建立检测鸡组织中氨苄西林残留量的高效液相色谱荧光法。[结果]氨苄西林在25～1000μg／kg浓度范围内，色谱峰面积与浓度呈线性关系，且线性关系良好。当氨苄西林添加量为10．0～125．0μg／ml时，鸡组织中AMP的平均回收率为71．23％～88．86％，相对标准偏差均低于10．74％；检测限和定量限分别为3．5（S／N=3）和10．0μg／kg（S／N=10）。[结论]该方法操作简便快速，可适用于鸡组织中氨苄西林的提取和检测，且准确度和精密度均符合残留分析的要求。[ Objective ] The research aimed to provide scientific basis for establishing the standards for detecting the residue of ampicillin （AMP） in chicken tissues. [ Method] A high performance liquid chromatography with fluorescence detection （HPLC-FLD） method was estab＊ lished for detecting the residual amount of ampicillin （AMP） in chicken tissues. [ Result] The chromatographic peak area showed a good linear relationship with ampicillin concentration in the range of 25 - 1 000 μg/kg. When the addition amount of ampicillin was 10.0 - 125.0 μg/kg, the average recovery rate of AMP in chicken tissues was 71.23% - 88.86%, with RSD of less than 10.74%. Limit of detection （LOD） and limit of quantitation （LOQ） were 3.5 μg/kg （ S/N = 3 ） and 10 μg/kg（ S/N = 10）, respectively. [ Conclusion ] This HPLC-FLD method was simple and rapid, and it was suitable for the extraction and determination of ampicillin in chicken tissues. And the accuracy and precession could meet the demands of residual analysis.

关 键 词：高效液相色谱 氨苄西林 荧光检测法 

分 类 号：S851.347[农业科学—预防兽医学]