水稻胡麻叶斑病病原菌的培养特性  

Cultural characteristics of pathogenic fungi of rice brown spot

作　　者：彭陈[1] 王瑞 陈洪亮[1,3] 王俊伟[1] 袁艺[3] 郭士伟[1] 

机构地区：[1]江苏省农业科学院农业生物技术研究所/江苏省农业生物学重点实验室,江苏南京210014 [2]江苏省农业科技实业总公司,江苏南京210014 [3]安徽农业大学生命科学学院,安徽合肥230036

出　　处：《江苏农业学报》2014年第3期503-507,共5页Jiangsu Journal of Agricultural Sciences

基　　金：江苏省农业科技自主创新基金项目[CX(12)2024]

摘　　要：为了解水稻胡麻叶斑病病原菌的生长发育特点,从光照、pH、温度、碳源、氮源等方面对其菌丝生长及产孢条件进行了研究。结果发现:水稻胡麻叶斑病病原菌菌丝生长的最适培养基为PDA培养基,最适光照为12 h/12 h光暗交替,最适pH为9,最适温度为25℃,最适碳源为蔗糖,最适氮源为甘氨酸,同时在不同培养基﹑光照时间﹑碳源及氮源上的菌落形态均有差异。孢子萌发的最适光照为24 h全光照,最适温度为25℃,最适pH为9,最适碳源为可溶性淀粉,最适氮源为硝酸钾。The conditions of mycelial growth and spore generation of pathogenic fungi of Bipolaris oryzae were stud-ied, such as light, pH, temperature, carbon source and nitrogen source. The results showed that the myelia grew best un-der light dark alternate（12 h/12 h）, 25℃ on the PDA medium with pH 9, sucrose as carbon source, and glycine as nitro-gen source. The colony morphology varied under the conditions of different media, light, carbon and nitrogen sources. The condition of 24-h full light, 25℃, pH 9, soluble starch as carbon source, and potassium nitrate as nitrogen source was the best of spore germination.

关 键 词：水稻胡麻叶斑病菌 菌丝 孢子 

分 类 号：S435.111.43[农业科学—农业昆虫与害虫防治]