高效液相色谱-荧光法测定阿维菌素在稻田环境基质中的残留  

Determination of Abamectin Residues in Paddy Field Environmental Matrices by High Performance Liquid Chromatography with Fluorescence Detector

作　　者：宁赢 彭秧锡[1] 欧晓明[1,2,3] 梁骥[2,3] 张贵群[2,3] 刘义珂[2,3] 聂思桥[2,3] 任竞[2,3] 刘金胜[2,3] 傅强[2,3] 

机构地区：[1]湖南人文科技学院,湖南娄底417000 [2]湖南化工研究院国家农药创制工程技术研究中心,湖南长沙410014 [3]农用化学品湖南省重点实验室,湖南长沙410014

出　　处：《精细化工中间体》2014年第3期66-69,72,共5页Fine Chemical Intermediates

摘　　要：采用高效液相色谱一荧光检测器（HPLC—FLD）建立了阿维菌素在水稻植株、谷壳、糙米、土壤及田水中的残留检测方法。样品用乙腈提取，经液液萃取净化，浓缩定容后衍生化，HPLC—FLD检测，外标法定量。结果表明，在添加水平为0．01～0．50mg／kg时，阿维菌素在水稻植株、谷壳、糙米、土壤和田水中的平均添加回收率为79．13％～102．98％，相对标准偏差为3．10％～6．71％。该方法的最小检出量为0.2ng，在水稻植株、土壤、谷壳、糙米和田水中的最低检测浓度均为0．01mg／kg。A method for the determination of abamectin residue in paddy plants, husk, coarse rice, soil and water was established using high performance liquid chromatography with fluorescence detector （HPLC-FLD）. The samples were extracted with acetonitrile, cleaned derivatized after concentration and constant volume. up by liquid-liquid separation, and directly pre-column The analysis was performed by HPLC-FLD with an external standard. The results showed that the average recoveries of abamectin in rice plants, husk, coarse rice, soil and water were from 79.10% to 102.98%, and the relative standard deviations were from 3.10% to 6.71% when the spiked levels ranged from 0.01 mg/kg to 0.50 mg/kg. The LOD was 0.2 ng, and the LOQ was 0.01 mg/kg in paddy plants, husk, rice, soil and water.

关 键 词：高效液相色谱-荧光检测器 阿维菌素 稻田 残留 

分 类 号：O657.71[理学—分析化学]