GenBank数据库中黄麻EST-SSR标记的开发及其通用性评价  

Development and Universality Evaluation of EST-SSR Markers from GenBank in Jute

作　　者：张立武[1] 袁民航 何雄威 刘星[1] 方平平[1] 林荔辉[1] 陶爱芬[1] 徐建堂[1] 祁建民[1] 

机构地区：[1]福建农林大学作物科学学院/作物遗传育种与综合利用教育部重点实验室,福建福州350002

出　　处：《作物学报》2014年第7期1213-1219,共7页Acta Agronomica Sinica

基　　金：高等学校博士学科点专项科研基金新教师类资助课题(20133515120002);国家现代农业产业技术体系建设项目(CARS-19-E06);农业部东南黄红麻实验观测站(农科教发2011);国家自然科学基金项目(31000734)资助

摘　　要：从GenBank公共数据库中下载黄麻表达序列标签838条,利用SSRPrimer软件对其进行SSR位点查找,利用Primer 3.0软件设计66对SSR引物,通过琼脂糖凝胶研究这些SSR引物的PCR扩增特点,以检测其多态性。结果表明,66对SSR引物在黄麻属6个不同类型材料的扩增中,42(63.6%)对引物至少在2个材料之间存在多态性。(AT)n重复基元和(GC-)n丰富的三核苷酸重复基元多态性较高,可作为黄麻SSR标记引物设计的首选。黄麻EST-SSR标记开发效率较高,不仅可以丰富黄麻分子标记的数量,而且为剖析黄麻重要性状的遗传机制奠定基础,这对于黄麻的遗传基础研究具有重要应用价值。In this study, a total of 838 jute expressed sequence tags （EST） were derived from Genl3ank. Using software Primer 3.0, 66 pairs of primers were designed based on the simple sequence repeat （SSR） sites screened by the online program SSRPrimer. These primers were amplified in six jute accessions of different types to test their polymorphism. The agarose gel electrophoresis results showed that 42 pairs of primers （63.6%） were polymorphic between at least two accessions and the PCR products were clear and stable fragment. These polymorphic EST-SSRs contained rich motif （AT）n or （AC-）n, which could be considered as the dominant motif in jute genome. Therefore, it is effective to develop SSR markers in jute from ESTs deposited in GenBank. The EST-SSRs developed will not only facilitate genetic research in jute （Corchorus spp.）, but also enrich molecular markers of jute genome, which is the basis for various studies in molecular biology.

关 键 词：黄麻 EST SSR 标记开发 多态性 

分 类 号：S563.4[农业科学—作物学]