国内外蚕豆核心种质SSR遗传多样性对比及微核心种质构建  

Genetic Diversity Analysis of Germplasm Resources and Construction of Mini-core Collections for Vicia faba L. at Home and Abroad

作　　者：姜俊烨 杨涛[1] 王芳[1] 方俐[1] 仲伟文 关建平[1] 宗绪晓[1] 

机构地区：[1]中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程,北京100081 [2]甘肃农业大学生命科学技术学院,甘肃兰州730070

出　　处：《作物学报》2014年第7期1311-1319,共9页Acta Agronomica Sinica

基　　金：科技部科技支撑项目(2013BAD01B05);科技部国际科技合作项目(2010DFB33340);国家自然科学基金项目(31101198);农业部作物种质资源保护项目(NB2013-2129135-25)资助

摘　　要：运用24对SSR引物,对国内外1075份初级地理蚕豆核心种质的遗传多样性分析显示,等位变异数、有效等位变异数及Shannon’s信息指数分别为8.54、2.26和1.02;对全部参试资源进行聚类分析,没有发现明显的群体结构,表明初级地理核心种质的代表性较好,遗传背景较广泛。之后采用每个类内随机抽样的方法构建含有129份国内资源和63份国外资源的蚕豆微核心种质,等位基因变异数、有效等位变异数和Shannon’s信息指数的保留比例分别为87.32%、101.26%和101.82%;经t检验得出微核心种质与全部参试资源群体间遗传多样性差异不显著,表明构建的微核心种质的遗传多样性可以代表初级地理蚕豆核心种质。The genetic diversity of 1075 genotypes from a primary geographic core collection of faba bean （Ficiafaba L.） was analyzed by using SSR markers. The number of observed and effective alleles of 8.54 and 2.26, the Shannon＇s information index of 1.02, were detected. Unconspicuous population structure among the 1075 genotypes indicated an extensive genetic background and nice representativeness. A mini-core collection comprised 129 Chinese and 63 oversea faba bean genotypes was randomly sampled from each cluster of dcndrogram including all the 1075 tested genotypes. The observed alleles, effective alleles and Shannon＇s information index of the mini-core collection covered 87.32%, 101.26%, and 101.82% of the primary geographic core collection respectively. The t-test did not show significant differences in genetic diversity between the primary geographic core collection and the mini-core collection, which validated the representativeness of the mini-core collection in genetic diversity of faba bean. The mini-core collection will play an important role in exploration of new genes within faba bean.

关 键 词：SSR 蚕豆 遗传多样性 核心种质 微核心种质 

分 类 号：S643.6[农业科学—蔬菜学]