硅胶色谱柱的亲水作用保留机理及其影响因素  

Hydrophilic interaction chromatographyon silica column:retention mechanism and its influential factors

作　　者：李瑞萍[1,2] 袁琴[1] 黄应平[1,2] 

机构地区：[1]三峡库区生态环境教育部工程研究中心,三峡大学,湖北宜昌443002 [2]三峡地区地质灾害与生态环境湖北省协同创新中心,湖北宜昌443002

出　　处：《色谱》2014年第7期675-681,共7页Chinese Journal of Chromatography

基　　金：国家自然科学基金项目(20977058);国家水体污染控制与治理科技重大专项(2012Zxo7104-002-02)

摘　　要：亲水作用色谱(HILIC)是替代反相色谱(RPLC)分离强极性及亲水性化合物的另一色谱模式,其分离机理与RPLC有很大不同,具有和RPLC互补的选择性。在HILIC模式中,采用正相色谱(NPLC)中的极性固定相及含高浓度有机溶剂(通常为乙腈)的水溶液为流动相。硅胶是开发最早、研究最为深入及应用最为广泛的HILIC固定相,本文介绍了硅胶色谱柱的HILIC保留机理,详细概述了操作条件如硅胶柱类型、流动相组成及柱温对HILIC分离的影响,并对硅胶填料色谱柱的HILIC模式的发展方向与应用前景进行了展望。Hydrophilic interaction chromatography（HILIC）is a valuable alternative to reversed phase liquid chromatography（RPLC）for the analysis of highly polar and hydrophilic compounds,in which the separation mechanism is quite different from RPLC and the separation selectivity is complementary to RPLC.This separation mode can be characterized as normal phase liquid chromatography（NPLC）on polar columns in aqueous-organic mobile phases rich in organic solvents（usually acetonitrile）.Silica has been the earliest developed and most widely used HILIC stationary phase.This review deals with the recent advances in the development of the retention mechanism on silica column with special attention to the effects of stationary phase,mobile phase composition and temperature on separation in HILIC mode.Moreover,the developing trends and applications of this HILIC mode are presented.

关 键 词：亲水作用色谱 保留机理 硅胶柱 流动相组成 柱温 

分 类 号：O658[理学—分析化学]