高效液相色谱-串联质谱法同时测定辽东楤木不同部位中5种皂苷类成分的含量  

Simultaneous determination of five triterpenoid saponins in different parts of Aralia elata byhighperformance liquid chromatography-tandem mass spectrometry

作　　者：马慧[1] 宋少江[2] 刘菲菲[2] 张艳[2] 彭缨[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016 [2]沈阳药科大学中药学院,辽宁沈阳110016

出　　处：《色谱》2014年第7期762-766,共5页Chinese Journal of Chromatography

基　　金：国家自然科学青年科学基金项目(81302661)

摘　　要：建立了高效液相色谱-串联质谱法（HPLC-MS/MS）用于测定辽东楤木不同部位中的楤木皂苷Ⅱ、楤木皂苷Ⅳ、楤木皂苷Ⅴ、楤木皂苷Ⅹ和楤木叶皂苷Ⅱ的含量,并比较了楤木不同部位中上述5种皂苷的含量差异。以Alltima C18柱（250 mm×4.6 mm,5μm）为分析柱,以乙腈和0.1%（体积分数）甲酸水溶液为流动相,梯度洗脱,流速为0.8 mL/min。在电喷雾正离子多反应监测（MRM）模式下进行检测。结果表明该方法中楤木皂苷Ⅱ、楤木皂苷Ⅳ、楤木皂苷Ⅴ、楤木皂苷Ⅹ和楤木叶皂苷Ⅱ分别在0.17-108μg/L、0.53-329μg/L、0.77-480μg/L、0.77-480μg/L和0.82-510μg/L范围内线性关系良好,相关系数（r^2）均大于0.999,提取回收率为99.0%-100.2%。该方法简单、快速、灵敏,可用于测定辽东楤木药材不同部位中的皂苷类成分的含量。测定结果发现楤木不同部位中上述5种皂苷总量多少的顺序为根皮〉叶〉种子〉芽。A method was developed for the simultaneous determination of congmunosideⅡ,congmunosideⅣ,congmunosideⅤ,congmunosideⅩand congmuyenosideⅡin different parts of Aralia elata by high performance liquid chromatography-tandem mass spectrometry（HPLC-MS/MS）.The chromatographic separation was performed on an Alltima C18analytical column（250 mm×4.6mm,5μm）using the mobile phases of acetonitrile and 0.1%（v/v）formic acid aqueous solution with gradient elution.The separated compounds were detected in multiple reaction monitoring（MRM）mode via positive electrospray ionization（ESI＋）.The linear ranges of congmunosideⅡ,congmunosideⅣ,congmunosideⅤ,congmunosideⅩ and congmuyenosideⅡ were 0.17-108μg/L,0.53-329μg/L,0.77-480μg/L,0.77-480μg/L and 0.82-510μg/L,respectively.The extraction recoveries varied from99.0%to 100.2%.The developed method is rapid,accurate and reproducible,and is successfully utilized as a quality control method for Aralia elata.The results indicate that the order of contents of these five saponins in different parts of Aralia elata is root barkleavesseedsbuds.

关 键 词：液相色谱-串联质谱法 皂苷 辽东楤木 

分 类 号：O658[理学—分析化学]