红豆杉无性繁殖关键技术研究  

Study on the Critical Techniques of Vegetative Propagation for Chinese Yew

作　　者：付秀琴[1] 

机构地区：[1]成都农业科技职业学院,成都611130

出　　处：《重庆师范大学学报（自然科学版）》2014年第4期150-154,共5页Journal of Chongqing Normal University:Natural Science

基　　金：四川省科技厅项目(No.2011HH0011)

摘　　要：红豆杉（Taxus wallichiana var.chinensis）属于国家珍稀濒危保护植物,野生资源保护压力巨大,同时又具有园林绿化和药用价值,市场开发前景广阔,研究红豆杉扦插等快速繁育技术是当前该物种保护和利用的关键任务之一。本研究采用小区试验方法,从扦插季节选择、插穗长度、生根剂使用方法、扦插床基质等方面对红豆杉扦插技术进行了研究,筛选出了适宜的红豆杉扦插枝条顺利生根存活的关键环节：在3月份插枝条尚未萌动前,以珍珠岩为扦插基质、将中国红豆杉剪成长度为5-10 cm的扦插枝条,用3 000 mg·kg-1的吲哚丁酸（IBA）浸泡插条处理。上述处理能够保障扦插枝条的愈伤组织形成率、须根数、根长度和根粗壮程度方面具有一定的优势,可以在生产实践上进行参考。The Chinese yew （Taxus wallichiana var. chinensis） is one of the trees that are listed as national rare and endangered plants. With limited wild resources and shrinking natural population size, it is a great challenge to implement conservation of these tree species without artificial facilitated propagation techniques. Being a garden plant with both medicinal and ornamental impor- tance, to propagate the plants rapidly from vegetative organs, such as, cutting is a promising way to safeguard the sustainable pro- tection and utilization of the Taxus resources and has broad marketing prospects as well. In order to find a combination of treat- ments that is suitable for the propagation of Chinese yew from cutting, we conducted a serial of field plot experiments, which includ- ed the selection of cutting applying seasons, lengths of cutting, agents of rooting promotion, and media of cutting beds. The results showed that higher rate of callus formation, maximum amount of roots, longer and vigorous roots could be obtained with the treat- ments of culturing the cutting in March before the sprouting of shoots, using the perlits as medium of cutting beds, 5--10 cm of the cutting length, and pre-treating the cuttings buy soaking in IBA solution with concentration of 3 000 mg·g-1. Hence we conclude that by application of the combination of these treatments when will result in more high quality seedlings. making vegetative propagation of Chinese yew in mass production

关 键 词：红豆杉 扦插 生根 愈伤组织 保护生物学 

分 类 号：Q949.95[生物学—植物学] Q945.51