马大杂种相思腋芽高效组培增殖体系  

An Efficient Axillary Bud Multiplication System of Acacia mangium × A. auriculiformis

作　　者：施琼[1] 胡峰[2] 黄烈健[1] 詹妮[1] 

机构地区：[1]中国林业科学研究院热带林业研究所,广州510520 [2]厦门大学生命科学学院,厦门361005

出　　处：《林业科学》2014年第6期55-60,共6页Scientia Silvae Sinicae

基　　金：国家"十二五"科技支撑计划项目(2012BAD01B0402)

摘　　要：以马大杂种相思组培苗为研究对象，通过筛选最佳的增殖培养基，调整增殖时间，探讨芽体长度、继代次数对增殖的影响，增殖培养对生根的影响，建立马大杂种相思腋芽高效组培增殖体系。结果表明：将健壮的初代培养无菌芽剪成含1个腋芽的茎段，接入最佳增殖培养基（改良 MS ＋6-BA1.5 mg·L -1＋NAA0.1 mg·L -1＋Ac0.04 g·L -1，添加蔗糖3%和琼脂7 g·L -1）中连续培养7代，7次继代的平均增殖倍数为2.52，增殖时间为35天，单株平均繁殖系数为14.99，且7次继代增殖倍数变化小、增殖芽质量好。将增殖芽的茎段（含2～3个腋芽）接入1/2MS＋IBA1.0 mg·L -1＋ NAA0.5 mg·L -1（添加蔗糖3%和琼脂7 g·L -1）进行生根培养，15天生根率可达96.5%，移栽后存活率为95.0%。To build an efficient axillary bud multiplication system of Acacia mangium x A. auriculiformis,the aseptic plantlets of A. mangiumx A. auriculiformis were used to screen the optimal protocol for in vitro multiplication. The optimal multiplication medium and period,length of the sterile stems,subculture times,rooting and transplant survival rate were studied. The results showed that after being cultured 7 times on improved-MS＋ 0. 5 mg·L -16-BA＋0. 1 mg·L -1 NAA ＋0.04 g·L -1Ac（with combination of 3% sugar and 7 g·L -1 agar）,which was the best multiplication medium screened from the trial test,the average proliferation times of the stem with one axillary bud were 2. 52,and the propagation coefficient of the plantlet was around 14. 99 after 35 days. Moreover,the plantlets grew well and there was no obvious variation of the multiplication ratio. The aseptic stem with 2 or 3 axillary buds was inoculated to 1/2MS ＋IBA1. 0 mg·L -1 ＋NAA0. 5 mg·L -1 （ with combination of 3% sugar and 7 g·L -1 agar） for rooting culture,the rooting rate was 96. 5% in 15 day and the transplant survival rate was 95. 0%.

关 键 词：马大杂种相思 有效增殖倍数 单株繁殖系数 继代次数 腋芽增殖 

分 类 号：S718.46[农业科学—林学]