台湾杉遗传多样性的ISSR分析  

Genetic Diversity of Taiwania cryptomerioides Detected by ISSR Analysis

作　　者：李江伟[1] 杨琴军[1] 刘秀群[1] 季华[1] 张卓文[1] 

机构地区：[1]华中农业大学园艺林学学院园艺植物生物学教育部重点实验室,武汉430070

出　　处：《林业科学》2014年第6期61-66,共6页Scientia Silvae Sinicae

基　　金：国家自然科学基金项目(30901130)

摘　　要：采用ISSR分子标记技术,对台湾杉4个自然居群的遗传多样性水平和遗传结构进行研究。从100条引物中筛选出10条,得到78个清晰的扩增条带,其中多态性条带61个。POPGENE分析结果表明:台湾杉在物种水平(多态条带百分率PPB=78.21%,Nei’s基因多样性指数H=0.249 4,Shannon’s信息指数I=0.377 8)和居群水平(PPB=52.56%,H=0.178 3,I=0.267 7)均具有中等水平的遗传多样性。AMOVA分析表明,4个居群间出现一定程度的遗传分化,且遗传变异主要发生在居群内。UPGMA聚类和Mantel检测结果表明,居群间的地理距离与遗传距离之间不存在显著相关性(r=0.043 3,P=0.435 6)。根据台湾杉的遗传变异规律,提出有效的保护措施。The genetic diversity and genetic structure of four natural populations of Taiwania cryptomerioides were investigated using inter-simple sequence repeat （ ISSR） . Of the 100 primers screened,10 primers were selected and they generated 78 stable and distinct bands,among which 61 bands were polymorphic. The result of POPGENE indicated that T. cryptomerioides had a medium level of genetic variation at the species （ the percentage of polymorphic bands PPB =78. 21%,Nei’s gene diversity H=0. 249 4,Shannon’s information index I=0. 377 8） and population levels （PPB =52. 56%,H=0. 178 3,I=0. 267 7）. There was to a certain level genetic differentiation among four populations （GST：0. 285 1; Shannon ’s genetic differentiation： 29. 14%; AMOVA genetic differentiation： 28. 31%） ,and most of the genetic differentiation occurred within populations. The UPGMA clustering and Mantel test showed that there was no significant correlation between the geographical and genetic distance（r = 0.043 3,P = 0.435 6）. Based on these findings,we propose appropriate conservation strategies for this endangered species.

关 键 词：台湾杉 ISSR 遗传多样性 遗传结构 保护策略 

分 类 号：S718.46[农业科学—林学]