气相色谱法同时测定西洋参药材根中12种农药残留的含量  

Gas Chromatography Method for Simultaneous Determination of Twelve Pesticide Residues in Root of American Ginseng

作　　者：赵春杰[1] 侯哲[1] 袁丽华 国无双 王淼[1] 

机构地区：[1]沈阳药科大学药学院,辽宁沈阳110016 [2]河北省医疗器械与药品包装材料检验所,河北石家庄050051

出　　处：《沈阳大学学报（自然科学版）》2014年第3期193-197,共5页Journal of Shenyang University：Natural Science

基　　金：国家自然科学基金资助项目(39970910)

摘　　要：目的：建立西洋参药材根中12种农药残留的分离测定方法．方法：采用气相色谱法，HP-608弹性石英毛细管柱，ECD检测器，柱温起始温度150℃，以5℃／min速度升温，至230℃，保温5min；进样器温度240℃，检测器温度250℃，载气为高纯氮，柱流速20mL／min，层吹流速40mL／min，用外标法计算农药残留的含量．结果：12种农药的峰面积和质量浓度（mg／L）在测定范围内均具有良好的线性关系，精密度良好，平均回收率为94．1％～99．4％．结论：所用方法简单准确，灵敏度高，适用于西洋参药材根中12种农药残留含量的同时测定．Objective： To establish a gas chromatography method for the determination of twelve pesticide residue in the root of American ginseng. Methods： Pesticide residue in the root of American ginseng was determined by direct injection on a HP-608 elastic quartz capillary column with a ECD detector. The initial temperature of the column was 150 ℃ ,with the increase rate of 5 ℃/min to 230 ℃ （maintaining for 5 min）. The temperature of injection was 24-0 ℃, the temperature of the detector was 250 ℃, the carrier gas was nitrogen gas and the rate was 20 mL/min, layer blown flow rate was 40 mL/min. The content of pesticide residues were calculated by external standard method. Results： The mass concentration and the peak area of twelve pesticide residues showed good linearity and specific, the precision was good and the average recoveries were 94. 1%-99. 4%. Condusion： The methods is simple, accurate and sensitive, this method can be used for detection of the twelve pesticide residues in the root of American Ginseng.

关 键 词：西洋参 气相色谱法 农药 残留量 含量测定 

分 类 号：O657.71[理学—分析化学] R917[理学—化学]