丹参酮IIA对TGF-β_1诱导的人肾小管上皮细胞增殖及Ⅰ型胶原分泌的影响  

Effect of Tanshione IIA on proliferation of human renal epithelial and secretion of collagen Ⅰ induced by TGF-β_1

作　　者：赵斐[1] 秦英[1] 高永红[2] 李俊辉[3] 张杰[1] 

机构地区：[1]北京中医药大学东直门医院肾病内分泌科,北京100700 [2]北京中医药大学东直门医院中医内科重点实验室,北京100700 [3]北京中医药大学东直门医院骨科,北京100700

出　　处：《上海中医药杂志》2014年第6期96-98,102,共4页Shanghai Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30973737)

摘　　要：目的探讨丹参酮IIA在防治肾间质纤维化方面的作用。方法将体外正常培养的人肾小管上皮细胞(HK-2)分为空白对照组、转化生长因子-β1组(TGF-β1,10μg/L)、干预一组(丹参酮IIA 1 mg/L+TGF-β110μg/L)、干预二组(丹参酮IIA 5 mg/L+TGF-β110μg/L)及干预三组(丹参酮IIA 10 mg/L+TGF-β110μg/L),分别予相应的干预措施。采用MTT方法检测HK-2的增殖以及用倒置相差显微镜观察细胞形态的变化;采用ELISA法检测上清液中Ⅰ型胶原的含量。结果 TGF-β1组(10μg/L)能刺激肾小管上皮细胞增殖以及促进细胞的形态向成纤维细胞发生转变;加入丹参酮IIA(1 mg/L^10 mg/L)共同作用后,细胞的增殖受到抑制,细胞形态逐渐趋向于正常,呈现明显的剂量依赖性。TGF-β1(10μg/L)能刺激肾小管上皮细胞Ⅰ型胶原的分泌增加,与空白对照组比较差异有统计学意义(P<0.05),与丹参酮IIA(1 mg/L^10 mg/L)共同作用后Ⅰ型胶原的表达量下降,并呈明显的剂量依赖性。结论丹参酮IIA能够维持HK-2细胞形态的稳定性,防止细胞向成纤维细胞方向发展,在一定程度上抑制了TGF-β1诱导的细胞增殖及分泌Ⅰ型胶原的作用,这可能也是其治疗肾间质纤维化的作用机制。Objective To explore the effect of Tanshione IIA in preventing and treating renal interstitial fibrosis. Methods Human renal tubular epithelial （ HK- 2 ） cells were cultivated in vitro and divided into five groups： blank control group, TGF-β1 10 μg/L group, Tanshione IIA groups （low, medium, high dose） , and each group was treated with corresponding drugs and reagents. The proliferation of HK-2 was detected by MTT and morphological changes of cells were observed by inverted phase contrast microscope. The content of collagen I was measured by ELISA. Results In TGF-131 10 μg/L group, HK-2 cells were stimulated by TGF- β1 and can be converted to fibroblast ceils. After combined with Tanshione IIA, cells proliferation was restrained and the cells morphology were tending to the normal gradually. It was showed a significant dose-dependent. Secretion of collagen [ was stimulated by TGF-β1 , with a significant difference between blank control group and TGF-β1 group （P 〈 0.05 ）. After combined with Tanshione IIA, expression of collagen I was reduced, with dose-dependent manner. Conclusion Tanshione IIA can maintain the morphological stability of HK-2 cells, prevent cells develop into fibroblast cells, and inhibit cell proliferation and secretion of collagen I induced by TGF-131 . Its mechanism may be association with the preventing and treating renal fibrosis by Tanshione IIA.

关 键 词：丹参酮IIA 转化生长因子Β1 人肾小管上皮细胞 Ⅰ型胶原 

分 类 号：R285.5[医药卫生—中药学]