夏枯草口服液中两种有效成分的HPLC法测定  

HPLC simultaneous determination of two effective components in “Xiakucao Oral Solution”

作　　者：章民军 张蔷蓉 胡侃 魏茂陈 

机构地区：[1]浙江省舟山市普陀区朱家尖街道卫生服务中心,浙江舟山316111 [2]浙江省温州市人民医院,浙江温州325000 [3]贵阳新天药业股份有限公司,贵州贵阳550018

出　　处：《上海中医药杂志》2014年第6期107-109,共3页Shanghai Journal of Traditional Chinese Medicine

基　　金：贵州省中药现代化科技产业开发专项[黔科合中药字(2013)5030号]

摘　　要：目的 建立同时测定夏枯草口服液中咖啡酸和迷迭香酸的高效液相色谱（HPLC）法。方法 检测条件：采用C18柱（4.6 mm×25 mm,5μm）,甲醇-0.1%甲酸梯度洗脱,流速1.0 ml/min,检测波长为330 nm,柱温为30℃。结果 咖啡酸在0.056-0.728μg范围内线性良好（r=1.0）,迷迭香酸在1.65-21.45μg范围内线性良好（r=1.0）,咖啡酸平均回收率为101.2%,RSD=1.33%（n=6）;迷迭香酸平均回收率为98.8%,RSD=1.31%（n=6）。结论 HPLC方法可用于同时测定夏枯草口服液中有效成分咖啡酸和迷迭香酸。Objective To establish the HPLC method for simuhaneous determination of the caffeic acid and rosmarinic acid in ＂Xiakucao Oral Solution＂. Methods Detecting condition： C18 column （4.6 mm × 250 mm, 5 μm） and the gradient of MeOH-0.1% acetic acid solution was taken as the mobile phase at a flow rate of 1.0 ml/min, the detection wavelength was 330 nm and column temperature at 30 ~C. Results The linear ranges of caffeic acid and rosmarinic acid were 0. 056 -0. 728μg （r = 1.0） and 1.65 ~ 21.45μg （r = 1.0） respectively. The average recovery rate of caffeic acid and rosmarinic acid were 101.2% and 98.8% respectively （ RSDs were 1.33% and 1.31% respectively, n = 6）. Conclusion The experimental results showed that the method could be used to determine the effective components including caffeic acid and rosmarinic acid in ＂Xiakucao Oral Solution＂.

关 键 词：夏枯草口服液 咖啡酸 迷迭香酸 高效液相色谱法 

分 类 号：R286.0[医药卫生—中药学] O657.72[医药卫生—中医学]