甜瓜α-甘露糖苷酶基因家族的全基因组分析  

Genome-wide analysis of α-mannosidases family genes in Cucumis melo

作　　者：乌斯呼吉日嘎拉 赵爱奇 莘少红 郝金凤[1] 哈斯阿古拉[1] 

机构地区：[1]内蒙古大学生命科学学院/内蒙古自治区牧草与特色作物生物技术重点实验室,内蒙古呼和浩特010021

出　　处：《广东农业科学》2014年第12期144-149,155,F0003,共8页Guangdong Agricultural Sciences

基　　金：国家自然科学基金(31101559)

摘　　要：α-甘露糖苷酶(α-mannosidases,α-Man)是生物界广泛存在的一种糖苷水解酶。通过已公布的甜瓜基因组数据库预测α-Man基因家族成员,其蛋白序列都含有完整的Glyco_hydro_47或Glyco_hydro_38保守结构域。再将筛选出的成员与拟南芥、大豆、番茄、可可树、葡萄、黄瓜、马铃薯、谷子的相应基因编码的蛋白序列进行进化分析比对,建立系统发生树和保守基序的预测分析。结果表明,甜瓜基因组中含有8个α-man基因,分为Ⅰ类和Ⅱ类,并分成5个亚族。同一类成员的蛋白结构域具有较高相似性与保守性,并且大部分成员基序的顺序和种类相同,不同类的成员之间基序差距较大。对甜瓜α-甘露糖苷酶基因家族的系统分析鉴定,有助于甜瓜α-甘露糖苷酶基因克隆和功能的进一步研究,为研究其他植物α-甘露糖苷酶基因家族的功能和进化提供参考依据。α-mannosidases （α-Man）, one group of glycosidases, have been universally distributed among most types of living organisms. Based on the published database of melon （Cucumis melo）, we found the α-Man family genes containing complete Glyco_hydro_47 or Glyco_hydro_38 domain. These protein sequences were analyzed by muhi-sequence aligned, which are similar in Arabidopsis thaliana, Glycine max, Solanum lycopersicum, Theobroma cacao, Vitis vinifero, C. sativus, S. tuberosum and Setaria italic. And the structure evolution relation and conserved motifs were researched by bioinformatics method. The results indicated that the melon genome containd 8 α-Man genes divided into class I and class II or 5 subfamilies. This protein was highly conservative with domain in the same class. Moreover, most of them had the same motifs in same order. On the contrary, the members of different classes had less similarity each other. This study is helpful for cloning and clarifying the function of α-Man genes in melon, and can provide the diverse reference of the function and evolution for α-mannosidase gene family in other plants.

关 键 词：甜瓜 α-甘露糖苷酶 进化分析 生物信息学 

分 类 号：Q941[生物学—植物学]