3个不同月季品种的组培技术研究  

Study on Tissue Culture of 3 Kinds of Different Roses

作　　者：龚维红[1] 尤伟忠[1] 丁小晏[1] 

机构地区：[1]苏州农业职业技术学院,江苏苏州215008

出　　处：《现代农业科技》2014年第12期170-170,174,共2页Modern Agricultural Science and Technology

基　　金：江苏省高等学校大学生实践创新训练计划资助项目;苏州农业职业枝术学院院级资助项目(NSl115)

摘　　要：以3个不同月季品种‘黄和平’、‘莱茵黄金’和‘恩钿’的茎段为外植体进行组织培养,研究不同品种月季组培增殖和生根情况。结果表明:3个品种月季适合的增殖培养基均为MS+6-BA 1.0 mg/L+NAA 0.1 mg/L,增殖系数分别能达到4.75、4.24和6.25;‘黄和平’和‘莱茵黄金’适合的生根培养基为1/2MS+NAA 0.1 mg/L+300 mg/L活性炭,生根率分别达90.8%、88.9%,‘恩钿’适合的生根培养基为1/2MS+NAA0.2 mg/L+300 mg/L活性炭,生根率能达91%。Using 3 different rose cuhivars ＇Yellow Peace＇, ＇Pfalzer gold＇ and ＇En tian＇ stems as explants for tissue culture,the tissue culture of different varieties of rose was made to study the proliferation and rooting rate. The results showed that：the suitable medium of 3 rose varieties for proliferation was MS＋6-BA 1.0 mg/L＋NAA0.1 mg/L,the proliferation coefficient could reach 4.75,4.24 and 6.25,respectively;the suitable rooting medium of ＇yellow peace＇ and ＇Pfalzer gold＇ was 1/2MS＋NAA 0：l mg/L＋300 mg/L activated carbon,the rooting rate was 90.8%, 88.9%, respectively.the suitable rooting medium of ＇Entian＇ was 1/2MS＋NAA 0.2 mg/L＋300 mg/L activated carbon,the rooting rate could reach 91%.

关 键 词：月季 组织培养 品种 

分 类 号：S685.12[农业科学—观赏园艺]