谷子和玉米基因组多倍化进化比较分析  

Comparative Analysis of Paleopolyploidy Evolution in Genomes of Setariaitalicaand Zea mays

作　　者：张琼[1] 王振怡[1] 马雪莲[1] 聂林曼 汪厚龙 王金朋[1] 

机构地区：[1]河北联合大学生命科学学院基因组学与计算生物学研究中心,河北唐山063009

出　　处：《河南农业科学》2014年第6期10-15,共6页Journal of Henan Agricultural Sciences

基　　金：国家自然科学基金项目(31100913);河北联合大学科学研究基金项目(z201230;z201235);河北联合大学大学生创新性实验计划项目(X2013044)

摘　　要：以谷子和玉米为研究对象,基于比较基因组学,利用改进的基因同源共线性方法对其基因组结构和基因同源信息进行比对分析,确定了物种基因组加倍的规模和加倍后的进化规律。结果表明:谷子中有3 846对(10.9%)、玉米中有6 016对(18.5%)由多倍化产生的重复基因,它们之间的直系同源基因对为13 652对;通过同源共线基因间的同义核苷酸置换率分析证实,玉米与谷子不仅共同经历了一次古老的全基因组加倍,而且在约12个百万年前玉米独立发生了一次较近的全基因组加倍;加倍后产生的大量重复基因片段常分布在染色体末端;基因组进化分析发现,玉米基因组进化速率比谷子快1/14。Based on the comparative genomics and the improved homologous gene collinearity method, the genomic structure and gene homology information of C4 plant Setaria italica and Zea rnays were comparatively analyzed in order to determine the doubled scale of genomes and the evolution law of polyploidy. The result showed that 3 846 pairs of paralogs（10. 9%） in Setaria italica genome and 6 016 pairs of paralogs（18. 5%） in Zea mays genome were generated by whole-genome duplication（WGD）,and 13 652 pairs of orthologs were identified between them. The analysis of synonymous nucleotide substitution rate between homologous colinear genes indicated that Zea rnays and Setaria italica shared an ancient WGD, and a maize-specific WGD event occurred 12 million years ago. The duplicated genes often distributed near the ends of chromosomes. The analysis of genome evolution showed that the gene evolution rate was 1/14 faster in Zea rnays than in Setaria italica.

关 键 词：谷子 玉米 多倍化 重复基因 基因共线性 

分 类 号：S513[农业科学—作物学] S515