日本鹌鹑艾美耳球虫单卵囊分离及雏鹑扩增研究  

Eimeria uzura Single Oocyst Isolation and Amplification in Young Quails

作　　者：张敏 韩琼琼 李清州[3] 王芳 梁楠[5] 张龙现[2] 菅复春[2] 宁长申[2] 

机构地区：[1]焦作市动物疫病预防控制中心,河南焦作454003 [2]河南农业大学牧医工程学院,河南郑州450002 [3]河南省农业科学院农业经济与信息研究所,河南郑州450002 [4]河南新正好生物工程有限公司,河南郑州450002 [5]河南农业职业学院,河南中牟451450

出　　处：《河南农业科学》2014年第6期149-151,共3页Journal of Henan Agricultural Sciences

基　　金：国家现代农业产业技术体系建设专项(nycytx-39)

摘　　要：为获取大量纯种日本鹌鹑艾美耳球虫（E．uzura），采用饱和食盐水漂浮法收集某场鹌鹑的球虫卵囊，26℃恒温培养至孢子化后，对据形态学鉴定为E．uzura的分离株进行单卵囊分离和雏鹑扩增试验。结果显示，26℃条件下卵囊孢子化时间为14h；单卵囊感染7日龄雏鹑，其潜隐期6d，显露期8～10d；以200个卵囊感染7日龄雏鹑，其潜隐期为5d，显露期为11d。To obtain a large number of pure-bred E. uzura, quails＇ coccidia oocysts were collected using the saturated brine flotation method and cultured at 26℃. According to morphological identification, single sporulated oocyst of E. uzura was separated and amplified in young quails. The results showed that sporulation of oocysts occurred for 14 h at 26 ℃. seven-day-old artificially-reared coccidian-free quails were inoculated orally with 1 and 200 sporulated oocysts of E. uzura, the prepatent period was 6 d and 5 d,and discharge of oocysts continued for 8-10 d and 11 d, respectively.

关 键 词：日本鹌鹑艾美耳球虫 单卵囊分离 卵囊孢子化 扩增 

分 类 号：S855.9[农业科学—临床兽医学]