太原市肠道病毒A71型基因特征分析  

Genetic Characteristics of Enterovirus Type A71 in Taiyuan City

作　　者：王骥涛[1] 张勇[2] 张晓林[1] 左芝红[1] 李轶平[1] 檀晓娟[2] 张燕[2] 许文波[2] 

机构地区：[1]太原市疾病预防控制中心,山西太原030001 [2]中国疾病预防控制中心病毒病预防控制所,卫生部医学病毒学和病毒病重点实验室,世界卫生组织西太平洋区脊髓灰质炎参比实验室,北京102206

出　　处：《中国疫苗和免疫》2014年第3期226-229,共4页Chinese Journal of Vaccines and Immunization

基　　金：山西省卫生厅科学技术攻关项目(编号201302026);国家科学技术艾滋病和病毒性肝炎等重大传染病防治重大专项--病毒性传染病病原谱流行规律及变异研究(编号2013ZX10004202);国家重点基础研究发展计划(973计划:编号2011CB504902);国家自然科学基金(编号30900063;30901259)

摘　　要：目的研究太原市手足口病（Hand，Foot and Mouth Disease，HFMD）患者中，肠道病毒A71型（Enterovirus Type A71，EVA71）的分子进化特征。方法采集太原市HFMD患者的咽拭子和粪便标本，采用荧光定量逆转录-聚合酶链反应（Reverse Transcription—Polymerase Chain Reaction，RT—PCR）方法检测EV核酸。然后随机选取11株EVA71阳性标本进行衣壳蛋白（Capsid Protein）VP，编码区基因扩增、核苷酸序列测定和分析，与其他48株各基因型和基因亚型的EV71代表株构建基因亲缘性关系树。结果太原市11株EVA71均属于C4基因亚型C4a进化分支，但11株EVA71在VP1编码区核苷酸和氨基酸的同源性分别为95．7％～100．0％和99．0％～100．0％。在亲缘进化树中显示，这些EVA71处在不同的簇中，表现为11株EVA71至少属于3条病毒传播链。结论太原市流行的EVA71属于C4基因亚型C4a进化分支，并且存在多个传播链。与中国其他地区流行的EVA71在基因亲缘关系和流行时间关系上都很接近，说明太原市EVA71不是独立进化的，而是与中国其他地区流行的EVA71在共同进化。Objective To analyze the molecular evolution of enterovirus type A71 （ EVA71 ） in patients with hand, foot and mouth disease （HFMD） in Taiyuan city. Methods Real-time reverse transcription- polymerase chain reaction （RT-PCR） for EV nucleic acid detection was performed on samples collected by nasopharyngeal swabs and on stool samples from HFMD patients in Taiyuan city. Eleven EVA71 positive samples were randomly selected for gene amplification and nucleotide sequence detection and analysis of the VP1 coding region in order to build a genetic affinity relationship tree with 48 other strains of EVA71 representing various genotypes and sub-genotypes. Results The 11 EVA71 strains clustered within the C4a evolution branch of C4 subgenotype. Homology of nucleotides and amino acids of 11 strains were 95.7% - 100. 0% and 99.0% - 100.0% , respectively. The phylogenetic tree demonstrated that the ll strains of EVA71 in different clusters belonged to at least 3 virus transmission chains. Conclusions The EVA71 strains that circulated in Taiyuan city belonged to C4a evolution branch of C4 sub-genotype. There were various virus transmission chains co-existing. The EVA71 strains in Taiyuan had a close phylogenetic and chronological relationship with EVA71 strains circulating in other areas of China's Mainland in recent years. The Taiyuan EVA71 strains had coevolved with the EVA71 strains in other areas of China's Mainland.

关 键 词：肠道病毒A71型 传播链 分子进化 C4基因亚型 

分 类 号：R373.11[医药卫生—病原生物学]