HPLC法同时测定旱芹中木犀草素和旱芹素的含量  

Determination of Luteolin and Apigenin in Apium graveolen L. by HPLC

作　　者：李敏[1] 孙莲[1] 孟磊[1] 

机构地区：[1]新疆医科大学药学院,新疆乌鲁木齐830011

出　　处：《食品研究与开发》2014年第10期106-108,共3页Food Research and Development

摘　　要：建立HPLC法同时测定旱芹中木犀草素和旱芹素含量的方法。采用C1(8 150 mm×4.6 mm,5μm)柱,流动相为乙腈∶0.01 mol/L醋酸铵(pH=4.8)=40∶60,等强度洗脱,检测波长330 nm,柱温25℃,流速1.0 mL/min。木犀草素和旱芹素的质量浓度分别在0.93μg/mL^4.94μg/mL(r=0.999 1)及1.03μg/mL^5.06μg/mL(r=0.999 4)范围内与峰面积呈现良好的线性关系,平均加样回收率依次为99.7%(RSD为2.4%),99.0%(RSD为1.8%)。To establish a method for simultaneous determination of Luteolin and Apigenin in Apium graveolen L. by HPLC. The sepration was performed on an C18 column with acetonitrile 0.01 mol/L ammonium acetate （pH=4.8）=60∶40 as a mobile phase at a flow rate of 1.0 mL/min. The detection wavelength was 330 nm at 25℃. There were good linear relationships between the mass concentrations and the peak areas of luteolin and apigenin in the ranges of 0.93μg/mL-4.94μg/mL（r=0.999 1）and 1.03μg/mL-5.06μg/mL （r=0.999 4） respectively. The average recoveries were 99.7%with RSD 2.4%and 99.0%with RSD 1.8%respectively. The method was quick,simple and repeatable for the determination of Luteolin and Apigenin in Apium graveolen L.

关 键 词：旱芹 木犀草素 旱芹素 高效液相色谱法 Apium graveolen L 

分 类 号：O657.72[理学—分析化学] R284.1[理学—化学]