脯氨酰脯氨酸二肽的液相合成及表征  

Solution-phase synthesis and characterization of proline-proline dipeptide

作　　者：李运涛[1] 范娇娇[1] 沈文[2] 王凡[1] 邓李慧 

机构地区：[1]陕西科技大学化学与化工学院,陕西西安710021 [2]陕西科技大学生命科学与工程学院,陕西西安710021 [3]东华大学纤维改性国家重点实验室,上海201620

出　　处：《陕西科技大学学报（自然科学版）》2014年第4期71-74,共4页Journal of Shaanxi University of Science & Technology

基　　金：陕西省科技厅自然科学基金项目(2011JM2005);陕西科技大学校级自选科研项目(ZX10-21)

摘　　要：以脯氨酸为原料在液相中合成了脯氨酰脯氨酸二肽(Pro-Pro).通过正交试验对实验条件进行优化,确定了肽键形成的最佳温度为30℃、时间为20h、投料摩尔比为1∶1.2、催化剂为N-羟基苯并三氮唑(HOBT)、溶剂为二氯甲烷(DCM),产率达78%.产物经红外光谱(IR)和核磁共振氢谱(1 H NMR)进行结构表征,通过熔点测定和热重(TG)测试考察产物热性能.结果表明:IR中产物的C=O和C-N伸缩振动吸收峰分别出现在1 689cm-1和1 397cm-1处,均向高波数移动,说明产物中吡咯环具有较大张力;TG检测表明脯氨酰脯氨酸二肽的最高使用温度为155℃.Pro-Pro was synthesized in solution phase with proline as the starting material. The experimental condition was optimized by orthogonal test in this paper. The maximum yield 78% was obtained when reaction temperature was 30 ℃, time was 20 h, mole ratio was 1 ： 1.2 with HOBT as catalyzer and DCM as solvent. The structure of products was characterized by IR Spectrum and ^1H NMR,the thermal properties was analyzed by melting point and TG. Results showed：Pro-Pro as tertiary amides, C= O and C-N stretching vibration absorption peak move to high wave number 1 689 cm^-1 and 1 397 cm^-1 ,which indicated pyrrole rings in proline has larger tension and strengthen bond vibration. The maximum service temperature of Pro-Pro was 155 ℃ indicated by TG test.

关 键 词：脯氨酸 二肽 液相合成 结构表征 

分 类 号：O629.72[理学—有机化学]