贵州蛇足石杉不同部位石杉碱甲含量测定  

Determination of Huperzine A in the Different Parts of Huperzia serrata from Guizhou Province by HPLC

作　　者：何春萍[1,2] 何庭[1] 舒春月 蒋向辉[2,3] 佘朝文[2,3] 

机构地区：[1]怀化学院化学化工系,湖南怀化418008 [2]怀化学院民族药用植物资源研究与利用湖南省重点实验室,湖南怀化418008 [3]怀化学院药用植物与民族植物学湖南省高校重点实验室,湖南怀化418008

出　　处：《怀化学院学报》2014年第5期1-4,共4页Journal of Huaihua University

基　　金：湖南省科技计划重点资助项目"侗族药用植物资源及其利用研究"(2009FJ2008)

摘　　要：分别采取超声法、索氏法、酸浸法和煎煮法提取贵州产蛇足石杉整株的石杉总生物碱,用高效液相色谱(HPLC)法测定石杉碱甲含量,比较不同提取方法对石杉碱甲含量的影响.4种不同提取方法的石杉碱甲提取率为超声法>索氏法>酸浸法>煎煮法.超声法提取的不同部位和全株的石杉碱甲含量为叶(0.0430%)>茎(0.0322%)>全株(0.0249%)>根(0.0085%),表明在蛇足石杉中石杉碱甲主要分布在地上部分.The total alkaloids of the whole herb of Huperzia serrata from Guizhou province were extracted by ultrasound , Soxhlet , acid leaching and decoction methods , respectively , and the contents of huperzine A were determined using HPLC . The extraction rates of huperzine A of the four methods were compared , and the rates were as follow ：ultrasound＆gt; Soxhlet＆gt; acid leaching＆gt;decoction . The contents of huperzine A extracted by ultrasound method in the whole herb and different parts of H . serrata differed significantly ：leaf （0.0430% ） ＆gt; stem （0.0322% ） ＆gt; whole herb （0.0249% ） ＆gt; root （0.0085% ） , indicating that the huperzine A in H . serrata was mainly distributed in the aboveground parts .

关 键 词：蛇足石杉 石杉碱甲 高效液相色谱 提取方法 

分 类 号：R284.2[医药卫生—中药学]