经大鼠腰骶部鞘内置管给药技术的研究  

Research of Intrathecal Catheterization Via Lumbosacral Puncture in Rats

作　　者：邓亚南 刘艳芳[1] 陈建平[2] 张宇[1] 

机构地区：[1]山西医科大学,山西太原030001 [2]山西医学科学院 山西大医院

出　　处：《中国医学创新》2014年第18期33-35,共3页Medical Innovation of China

基　　金：山西省自然科技基金(2012011042-4);山西省回国留学人员科研资助项目(2011-045)

摘　　要：目的：改进大鼠经皮穿刺腰骶部鞘内置管技术，探索更加简便、快捷、安全、有效的大鼠鞘内给药的新方法。方法：健康雄性大鼠60只，按随机数字表法分为经寰枕膜鞘内置管组（C组，n=30）和经皮穿刺腰骶部鞘内置管组（T组，n=30）。戊巴比妥钠腹腔麻醉后，C组大鼠接受经皮切开寰枕膜鞘内置管操作，T组大鼠接受在L6-S1间隙，用20 G穿刺针行经皮穿刺腰骶部鞘内置管操作。两组大鼠在操作成功后，均经导管注入2%利多卡因10μL。观察并比较两组大鼠置管成功只数、瘫痪只数以及死亡只数。结果：与C组比较，T组大鼠鞘内置管成功率明显提高，术后瘫痪、死亡率明显减少，差异均有统计学意义（P〈0.05）。结论：经皮穿刺腰骶部鞘内置管技术是一项简便、快捷、安全、有效的鞘内给药方法。To explore a simple,safe,fast and effective method of intrathecal application by studying the method of intrathecal catheterization via lumbosacral puncture in rats.Method：60 healthy rats were randomly divided into the intrathecal catheterization through the atlantooccipital membrane group（the group C,n=30）and the intrathecal catheterization through lumbosacral puncture group（the group T,n=30）. All the rats in the two groups received abdominal anesthesia by pentobarbital natrium. The group C received intrathecal catheterization by the atlantooccipital membrane. The group T received intrathecal catheterization by 20 G needle in L6-S1 interspace. Then,all the rats in the two groups were administrated 2% lidocaine hydrochloride 10 μL via the catheter. The number of successful catheterization,paralysis and death were compared in the two groups.Result：Compared with the group C,the success rate of catheterization was significantly increased,the incidence of death and paralysis were significantly decreased in the group T,the differences were statistically significant（P〈0.05）.Conclusion：Intrathecal catheterization by lumbosacral puncture is an effective and safe intrathecal catheterization method for rats undergoing scientific experiments.

关 键 词：蛛网膜下腔 经皮穿刺 鞘内置管 大鼠 

分 类 号：R965[医药卫生—药理学]