自制双管套管在大鼠前扣带回皮层置管给药的方法  

The Cannulation and Administration Techniques with Home-made Double Cannula in the Anterior Cingulate Cortex in Rats

作　　者：冯晓璞 兰坤[1] 张肖怡[1] 宋瑞瑞[1] 王锐[1] 张宇[1] 

机构地区：[1]山西医科大学,山西太原030001

出　　处：《中国医学创新》2014年第18期42-45,共4页Medical Innovation of China

基　　金：国家自然科学基金资助项目(81371254)

摘　　要：目的:自制双管套管在大鼠脑部前扣带皮层双侧注射药物。方法:首先自制双管套管,在立体定位仪的指引下将双管套管精确定位埋置并插入堵丝,然后用牙科水泥和螺钉将其固定于颅脑顶部;待大鼠术后体重恢复,将连接有微量进样器的注射针插入已埋置好的套管,用微量注射泵将药物缓慢注射于两侧前扣带皮层区域;给药完毕后即可观察大鼠的行为反应;如此可反复多次给药进行长期实验观察。结果:药物被准确注射到目的脑区,即双侧前扣带皮层,实验过程中大鼠状态良好,行为反应如预期。结论:自制双管套管的方法简便易行、经济可靠,实验取得良好的效果。To catheter and administrate in bilateral anterior cingulate cortex（ACC）with home-made double cannula in rats in our experiment.Method：Firstly the double cannulas were made by ourselves,then under the guidance of the stereotaxic instrument,a double cannula was implanted in ACC region on the top of rats’skull,and was fixed with denture base resins and bolts. When the body weight of rats returned to normal after surgery,the needle connecting with the micro-injector was inserted into cannula,then the reagent was slowly pushed into ACC region by syringe pump. After the administration was finished,behavioral test could be started. Used this technique,local application of reagent in brain region might be repeated for many times. Result：The reagent was injected accurately into the bilateral ACC region. And during the experiment,the behavioral responses of rats were as desired. Conclusion：Home-made double cannula get a good effect in the experiment. The making process is simple,economic and reliable.

关 键 词：前扣带回皮层 双管套管 脑内置管 大鼠 

分 类 号：R96[医药卫生—药理学]