65份野生油茶种质遗传多样性的ISSR和RAPD标记分析  

Analyses of genetic diversity among 65 wild Camellia oleifera based on ISSR and RAPD

作　　者：考安都[1] 王述贵[1] 王艳芹[1] 徐洲[1] 周永红[2] 丁春邦[1] 

机构地区：[1]四川农业大学生命科学与理学院,四川雅安625014 [2]四川农业大学作物基因资源与遗传改良教育部重点实验室,成都611130

出　　处：《广西植物》2014年第3期419-425,共7页Guihaia

基　　金：四川省科技厅科技支撑计划项目(2011SZ0283)

摘　　要：利用ISSR和RAPD标记,对名邛台地野生油茶种质进行遗传多样性分析。从60条简单重复序列引物中筛选出16条引物,在65份样品中共扩增出213条带,其中多态位点为203个,多态位点百分率为95.31%;从30条寡居核苷酸引物中筛选出8条引物,共扩增出105条带,其中多态性位点94个,多态位点百分率为89.52%。结果表明:名邛台地野生油茶种质具有较丰富的遗传多样性,ISSR和RAPD标记可以应用于油茶种质遗传多样性分析。The genetic diversity among 65wild Camellia oleiferafrom Mingqiong platform were discussed.213loci were identified with 16simple sequence repeat primers screened from 60primers,out of which 203loci were polymorphic and the percentage of polymorphic bands was 96.31%;105loci were identified with 8oligonucleotide primers screened from 30primers,out of which 94loci were polymorphic and the percentage of polymorphic bands was 89.52%.The results showed that ISSR and RAPD markers could be used in genetic diversity analysis,C.oleifera had a high level of genetic diversity.

关 键 词：油茶 遗传多样性 ISSR RAPD 聚类分析 

分 类 号：Q949.93[生物学—植物学]