HPLC-Q-TOF/MS指纹技术对比分析附子配伍炙甘草前后化学组分变化  

Changes of chemical ingredients before and after compatibility of Aconiti Lateralis Radix Praeparata and Glycyrrhizae Radix et Rhizoma Praeparata cum Melle analyzed by HPLC-Q-TOF/MS fingerprint technology

作　　者：林彦君[1] 游宇[1] 季宁平[1] 章津铭[1] 陈秋薇[1] 傅超美[1] 

机构地区：[1]成都中医药大学,四川成都611137

出　　处：《中草药》2014年第11期1556-1560,共5页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金面上项目(81073070)

摘　　要：目的分析附子-炙甘草配伍前后化学组分变化,探讨附子配伍炙甘草减毒的机制。方法采用HPLC-Q-TOF/MS分别对附子配伍炙甘草前后汤液进行化学对比研究。分别制备附子单煎液与附子炙甘草合煎液供试品溶液,在同一分析条件下,分别建立其HPLC-MS指纹图谱,并采用Q-TOF/MS检测。结果从附子单煎液中指认出20种成分及其结构;从附子炙甘草合煎液中指认出32种成分及结构,其中4种成分来源于炙甘草,28种成分来源于附子,但附子配伍炙甘草前后汤液中生物碱成分种类和量差别均较大。结论说明配伍炙甘草可能导致汤液中附子生物碱成分状态的改变,为附子-炙甘草配伍减毒机制的探讨提供了实验依据。Objective To analyze the changes of chemical ingredients before and after compatibility of Aconiti Lateralis Radix Praeparata（ALRP） and Glycyrrhizae Radix et Rhizoma Praeparata cum Melle（GRRPM）,and to explore the possible mechanism of toxicity attenuation of their compatibility.Methods The chemical ingredients in the decoction of ALRP and the decoction of compatibility of ALRP and GRRPM were comparatively researched by HPLC-MS.Single decoction of ALRP and compound decoction of compatibility of ALRP and GRRPM were prepared,and their HPLC-MS fingerprints were respectively established and determined by Q-TOF/MS under the same condition.Results Twenty ingredients and their structures were identified from single decoction of ALRP; 32 ingredients and their structures were indentified from the decoction of ALRP and GRRPM,among them 4 from GRRPM,28 from ALRP.And the alkaloid categories and contents of ALRP were significantly different before and after the compatibility with GRRPM.Conclusion The compatibility with GRRPM could change the alkaloid composition in ALRP,which provides the experimental evidences for the toxicity attenuation mechanism of compatibility of ALRP and GRRPM.

关 键 词：附子 炙甘草 配伍 HPLC-Q-TOF MS 化学组成 指纹技术 

分 类 号：R286.02[医药卫生—中药学]