五味子的UPLC指纹图谱研究  

UPLC fingerprint of Schisandra chinensis

作　　者：刘芷[1] 贾英[1] 赵旭[1] 张晨宁[1] 吴博[1] 毕开顺[2] 

机构地区：[1]沈阳药科大学中药学院,沈阳市中药药效物质基础筛选与评价重点实验室,辽宁沈阳110016 [2]沈阳药科大学药学院,中药质量控制关键技术国家地方联合工程实验室,辽宁沈阳110016

出　　处：《中草药》2014年第11期1631-1633,共3页Chinese Traditional and Herbal Drugs

基　　金：辽宁省科学技术计划项目资助(2011412004-1);沈阳市科技计划项目(F12-153-9-00;F13-287-1-00)

摘　　要：目的建立五味子Schisandra chinensis的超高效液相色谱(UPLC)指纹图谱。方法采用Acquity UPLC HSS T3色谱柱(100 mm×2.1 mm,1.8μm);以乙腈-水为流动相梯度洗脱;体积流量0.5 mL/min;柱温30℃;检测波长216 nm;进样量2μL。结果首次建立了五味子的UPLC特征指纹图谱共有模式,标定了21个共有峰,结合保留时间和紫外光谱分析,指认了五味子醇甲、五味子醇乙、当归酰戈米辛H、五味子酯甲、五味子酯乙、五味子甲素、五味子γ-乙素、五味子乙素和五味子丙素的峰位。19批五味子的相似度均在0.970以上。结论该方法快速、高效,可用于五味子的质量评价。Objective To establish the UPLC fingerprint of Schisandra chinensis.Methods The chromatographic fingerprint was obtained with Acquity UPLC HSS T3 column（100 mm × 2.1 mm,1.8 μm） and gradient eluted with acetonitrile and water; The flow rate was 0.5 mL/min,and the column temperature was maintained at 30 ℃.The detection wavelength was set at 216 nm,and the injection volume was 2 μL.Results The common mode of UPLC fingerprint of S.chinensis was set up for the first time.There were 21 common peaks in the fingerprints.Schisandrin,schisandrol B,angeloylgomisin H,schisantherin A,schisantherin B,deoxyschizandrin,γ-schisandrin,gomisin N,and schisandrin C were identified by comparing the retention time and their ultraviolet spectra.The similarities of 19 batches of S.chinensis were above 0.970.Conclusion The method is fast and efficiency.It can be used for the quality evaluation of S.chinensis.

关 键 词：五味子 超高效液相色谱 指纹图谱 质量评价 木脂素 

分 类 号：R286.022[医药卫生—中药学]