华北落叶松成熟合子胚不定芽诱导及伸长研究  

Adventitious Buds Induction and Elongation from Mature Zygotic Embryo of Larix principis-rupprechtii Mayr.

作　　者：赵健[1] 李旦[2] 张金凤[1] 

机构地区：[1]林木育种国家工程实验室,林木花卉遗传育种教育部重点实验室,国家林业局树木花卉育种生物工程重点开放实验室,北京林业大学生物科学与技术学院,北京100083 [2]西南林业大学云南生物多样性研究院,昆明650224

出　　处：《中国农学通报》2014年第16期12-17,共6页Chinese Agricultural Science Bulletin

基　　金：教育部"长江学者和创新团队发展计划"(IRT13047);国家自然科学基金项目"科研训练及科研能力提高项目"(J1103516);北京市与中央在京高校共建项目"华北落叶松遗传资源收集评价及新品种选育"(GJ2011-2);国家林业局林业公益性行业专项"重要乡土树种核心种质评价及高效育种共性技术研究"(201004009)

摘　　要：本研究以华北落叶松成熟合子胚为外植体,进行华北落叶松离体器官发生的初步探究。试验采用不同消毒方法对华北落叶松成熟合子胚进行消毒,设计了以6-BA、NAA及蔗糖为试验因素的L934正交试验进行华北落叶松不定芽的诱导研究,并且在不定芽伸长试验中添加了少量活性炭。试验结果表明,(1)将种子在用流水冲洗12 h,75%酒精消毒3 min,再转入0.1%升汞消毒10 min所获得的综合效果较好;(2)光照条件下(约2000 lx),DCR培养基+4 mg/L 6-BA+0.1 mg/L NAA+40 g/L蔗糖为诱导不定芽的最佳组合,其中6-BA影响作用显著,NAA、蔗糖则无显著影响;(3)1.0 g/L活性炭对不定芽伸长有显著的促进作用,平均伸长达0.43 cm。以上研究为华北落叶松组培器官发生及进一步的遗传转化研究奠定了良好的理论与实践基础。In this study, mature zygotic embryos of Larixprincipis-rupprechtii Mayr. was used for a preliminary investigation on the organogenesis of Larix principis-rupprechtii Mayr.. Different disinfection methods were utilized to process with the explants and an orthogonal experiment designed with 6-BA, NAA and sucrose was employed to investigate the induction of adventitious buds. Moreover, a few activated carbons were added to the medium in the elongation phase. The results showed that （1） the optimal disinfection method was that after rinsed in running water for 12 hours, the seeds were surface decontaminated with 75% ethanol for 3 minutes and then disinfected with 0.1% mercuric chloride solution for 10 minutes; （2） The best combination of adventitious buds induction was DCR medium supplemented with 4 mg/L 6-BA, 0.1 mg/L NAA and 40 g/L sucrose under a light intensity of 2000 ix, in which NAA and sucrose were no significant effect; （3） 1.0 g/L activated carbon played a positive role in elongating of adventitious buds, which was 0.43 cm averageelongation. It is significantly theoretical and practical basis for the organogenesis and further genetic transformation research of Larix pgncipis-rupprechtii Mayr.

关 键 词：华北落叶松 成熟合子胚 外植体消毒 不定芽诱导与伸长 

分 类 号：S718.46[农业科学—林学] Q943.1[生物学—植物学]