NaOH处理对紫椴种子休眠解除及生理生化特性的影响  

Effects of NaOH Treatment on Breaking Dormancy and Physiological and Chemistry Characteristic of Tilia amurensis Rupr. Seeds

作　　者：郑金辉 林士杰[2] 张艳敏 王艳杰 林永红[4] 崔凤文[4] 姜中华 徐炳芳 王亚静 梁宏玉 

机构地区：[1]吉林省龙湾保护区管理局,吉林通化135102 [2]吉林省林业科学研究院,长春130033 [3]梨树县林业局,吉林四平136500 [4]吉林省临江林业局,吉林白山134600 [5]长春北方绿化中心,长春130119

出　　处：《中国农学通报》2014年第16期35-40,共6页Chinese Agricultural Science Bulletin

基　　金：吉林省科技发展计划资助项目"紫椴种子休眠解除与萌发过程中物质转化的研究"(20100579)

摘　　要：为研究NaOH处理打破紫椴种子休眠的效果及NaOH处理后对紫椴种子萌发过程中贮藏物质的影响,采用不同质量分数NaOH处理紫椴种子,结合变温层积,定期统计发芽势、发芽率,并在层积过程中定期随机取样进行贮藏物质的测定。结果表明:30%NaOH处理1.5 h对紫椴种子休眠解除效果最好,发芽势平均为38%,发芽率平均为71.66%,20%NaOH处理1.5 h及10%NaOH处理6 h效果次之。与对照相比,NaOH处理后种子在整个层积过程中,淀粉、可溶性蛋白和可溶性糖含量显著下降,淀粉酶活性变化剧烈,呈"W"型。说明NaOH处理能够有效地解除紫椴种子的休眠,同时有效地改善了种子的透水透气性,在一定程度上加速了紫椴种子休眠解除过程中生理生化反应的进程。The Tilia amurensis Rupr. seed without pericarp was researched by NaOH in order to discuss the mechanism of breaking dormancy, and the changes of storage substance content in the integrative treated. Germination potential, germination rate and storage substance content were determined during seed germination of Tilia amurensis Rupr. by socking in NaOH. The results showed, all of them could reach the goal, but the best method was treating with 30% NaOH for 1.5 h. The average germination potential and germination rate were 38% and 71.66%, 20% NaOH for 1.5 h and 10% NaOH for 6 h were the secondplace. Compared with control, the starch, soluble protein and soluble sugar declined rapidly after germination, and the amylase changed with ＂W＂ after treated by NaOH. NaOH treatments were used to break seed coat barrier and change reactions of physiology and bio-chemistry in Tilia amurensis Rupr. seeds effectively.

关 键 词：紫椴 种子休眠 NAOH 可溶性蛋白 淀粉 

分 类 号：S72[农业科学—林木遗传育种]