卷丹不同继代试管苗茎尖超低温保存效果及组织细胞学研究  

Effect of Successive Subculture Shoot Tips in Vitro of Lilium lancifolium on Cryopreservation

作　　者：沈琛[1] 徐瑾[1] 李秉玲[1] 刘燕[1] 

机构地区：[1]花卉种质创新与分子育种北京市重点实验室/国家花卉工程技术研究中心/北京林业大学园林学院,北京100083

出　　处：《中国农学通报》2014年第16期139-142,共4页Chinese Agricultural Science Bulletin

基　　金：国家自然基金项目"花粉超低温保存差异蛋白在氧化应激和细胞凋亡中作用研究"(31370693)

摘　　要：为了研究组培茎尖的继代次数对其超低温保存后存活的影响,以卷丹试管苗不同继代次数的茎尖为材料,采用玻璃化法进行了超低温保存效果比较研究。结果表明,1-4代试管苗茎尖冻后存活率分别为55.8%、93.3%、67.0%和64.1%。同时,即初代培养后继代培养2个月的第2代试管苗茎尖超低温保存后存活率显著高于其他继代培养子组培苗。对各代试管苗茎尖进行石蜡连续纵切片显微观察,结果表明2代试管苗顶端分生组织细胞形态不同于其他继代代数,其显著特征是细胞体积小、细胞核大且细胞质浓密,这可能是其茎尖超低温保存显著高于其他3代的原因。In order to study the effect of subculture times of shoot tips on cryopreservation, the survival rates of 1-4 successive subculture shoot tips in vitro of Lilium lancifolium after cryopreservation were compared. The results showed that the survival rates were 55.8%, 93.3%, 67.0% and 64.1% respectively. Meanwhile the second generation subcultures which had been sub-cuhured two months obtained the higher survival rate than other generations. However, continuous paraffin sections of shoot tips of every generation were investigated by microscopy, the results indicated the second generation plantlets was different from others in morphological studies of protomerism cell, which involved smaller cell volume, larger cell nuclear and richer cytoplasm, and this may lead to the higher survival rate of the second generation after cryopreservation than others.

关 键 词：卷丹 茎尖 继代次数 超低温保存 存活率 显微结构 

分 类 号：S682.2[农业科学—观赏园艺]