利用生物反应器大量生产东北刺人参不定根的研究  

Mass Production of Adventitious Roots of Oplopanax elatus in Bioreactor

作　　者：于丹[1] 朴炫春[1] 李阳[1] 魏楠楠[1] 廉美兰[1] 

机构地区：[1]延边大学长白山生物资源与功能分子教育部重点实验室,吉林延吉133002

出　　处：《中国农学通报》2014年第16期252-255,共4页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金"东北刺人参不定根反应器培养机制及抗氧化特性研究"(31260182)

摘　　要：为了大量生产东北刺人参不定根,研究了培养方式和反应器内培养基中IBA浓度、糖种类以及白糖浓度对其不定根增殖生长的影响,结果表明生物反应器可大量生产东北刺人参不定根;在反应器培养时,MS培养基中添加3 mg/L的IBA时不定根长势较好,其鲜物重、干物重最大,分别达到378.3、34.8 g;而白糖和蔗糖对不定根的增殖效果显著好于葡萄糖和果糖,因此,选用低价的白糖作为碳源,白糖浓度为50 g/L时其鲜物重、干物重以及增殖系数达到最大,分别为551.9 g、57.2 g和26.6。For mass production of adventitious roots of Oplopanax elatus Nakai, the effects of culture methods, IBA concentrations, sugar types and white sugar concentrations during bioreactor on adventitious roots proliferation and growth were studied. The results adventitious roots proliferation was observed when 3 showed that bioreactor culture was optimal. The best mg/L IBA was added into the medium during bioreactor culture, the fresh and dry weight reached the maximum （378.3, 34.8 g）. White sugar and sucrose than glucose and fructose in adventitious roots proliferation, therefore the cheap white sugar was were better regarded as carbon source, and white sugar concentration of 50 g/L was the most suitable for fresh and dry weight as well as multiplication coefficient, the maximum were 551.9 g, 57.2 g and 26.6, respectively.

关 键 词：东北刺人参 不定根 生物反应器 增殖 

分 类 号：S-9[农业科学]