抑制小桐子溃疡病内生细菌的筛选  

Screening of Endophytic Bacteria Inhibiting Canker of Jatropha curcas

作　　者：郭建伟[1] 吕相贤 杨建[1] 杨会敏[1] 高源[2] 李成云[2] 

机构地区：[1]红河学院生命科学与技术学院、云南省高校农作物优质高效栽培与安全控制重点实验室,云南蒙自661100 [2]云南农业大学植物病理重点实验室、农业生物多样性与病害控制教育部重点实验室,云南昆明650201

出　　处：《云南农业大学学报（自然科学版）》2014年第4期610-613,共4页Journal of Yunnan Agricultural University:Natural Science

基　　金：红河学院硕士点植物保护一级学科建设项目;2012年度红河学院大学生科技创新基金(SSTIF)项目(SZ1209)

摘　　要：筛选抑制小桐子溃疡病的内生细菌,为进一步筛选促生长及抗病内生细菌奠定基础。利用组织破碎、稀释涂布法及对峙培养法从小桐子根、茎、叶筛选抑制小桐子溃疡病的内生细菌,然后通过生理生化特征进行初步鉴定。从小桐子根、茎、叶分别获得15株、14株、16株内生细菌,表明小桐子内生细菌的分布具有一定的组织差异性;从根、茎、叶分别筛选到3株、6株、2株等11株拮抗菌以及5株、2株、3株等10株营养竞争菌,表明不同功能内生细菌的分布也具有一定的组织差异性。芽孢杆菌Wx-1和短芽孢杆菌Wx-4的相对抑菌率达35%以上,具有一定的潜在生防价值。To screen endophytic bacteria inhibiting canker of Jatropha curcas,so as to lay foundation for screening endophytic bacteria of growth-promoting and disease resistance.The endophytic bacteria inhibiting canker were separated by tissue crashing method,dilution coating method and confrontation culture method from root,stem and leaf of J.curcas,then bacteria above were identified through physiological and biochemical characteristics.15,14 and 16 strains of endophytic bacteria were obtained respectively from root,stem and leaf,which suggested that the distribution of endophytic bacteria had a certain difference in organization ; 3,6,2 strains antagonistic pathogen and 5,2,3 strains competing nutrient with pathogen respectively from root,stem and leaf,which suggested that the difference distribution of endophyte bacteria with difference function collaborative resisted the invasion of pathogenic Botrosphaeria fuliginosa.Bacillus Wx-1 and Brevibacillus Wx-4 identified by physiological and biochemical characteristics,which had a certain potential bio-control value because of t more than 35% of relative inhibitive ratio.

关 键 词：溃疡病 内生细菌 拮抗作用 营养竞争 

分 类 号：S476[农业科学—农业昆虫与害虫防治]