大鼠骨骼肌损伤后PMN、MNC及FBC百分率与损伤时间关系  

Correlation between Percentages of PMN, MNC, FBC and Wound Age after Skeletal Muscle Injury in Rats

作　　者：于天水[1,2,3] 官大威[3] 赵锐[3] 张海东[1,2] 百茹峰[1,2] 

机构地区：[1]司法文明协同创新中心,北京100088 [2]中国政法大学证据科学教育部重点实验室,北京100088 [3]中国医科大学法医学院,辽宁沈阳110001

出　　处：《法医学杂志》2014年第3期166-168,共3页Journal of Forensic Medicine

基　　金：国家自然科学基金青年科学基金资助项目(81202383);教育部高等学校博士学科点专项科研基金资助项目(20122104110025)

摘　　要：目的：研究大鼠骨骼肌机械性损伤后不同时间点多形核白细胞（polymorphonuclear leucocytes ， PMN）、单个核细胞（mononuclear cells，MNC）及成纤维样细胞（fibroblastic cells，FBC）百分率的变化。方法建立大鼠骨骼肌机械性损伤动物模型，随机分为伤后6h、12h、1d、3d、7d、10d、14d及正常对照组。应用HE染色法和图像分析检测大鼠骨骼肌损伤后不同时间点PMN、MNC及FBC百分率。结果伤后6~12 h，损伤区内可见PMN和MNC浸润，PMN百分率达到峰值；伤后1 d，损伤区内主要以MNC浸润为主，MNC百分率达到峰值，而PMN百分率开始下降；伤后3~7 d，FBC百分率开始逐渐增加，PMN和MNC百分率则逐渐下降；伤后10~14 d，FBC百分率达到峰值。结论大鼠骨骼肌损伤区内PMN、MNC及FBC百分率呈时间规律性变化，有望成为骨骼肌损伤时间推断的参考指标。Objective To study the percentages of polymorphonuclear leukocytes （PMN）, mononuclear cells （MNC） and fibroblastic cells （FBC） in different posttraumatic intervals after skeletal muscle mechanical injury in rats. Methods The rat model of skeletal muscle mechanical injury was established. The rats were divided into injured groups （6 h, 12 h, 1 d, 3 d, 7 d, 10 d and 14 d after injury ） and control group. The percentages of PMN, MNC and FBC in different posttraumatic intervals after skeletal muscle mechanical injury were assessed with HE staining and image analysis. Results At postinjury 612 h, the percentages of PMN and MNC infiltration appeared in injured sites and that of PMN reached peak. At 1 d, the percentage of MNC infiltration appeared and reached peak, while that of PMN decreased. At 37 d, the percentage of FBC gradually increased, while that of PMN and MNC decreased. At 1014 d, the percentage of FBC reached peak. Conclusion The percentages of PMN, MNC and FBC in injured zones showed timedependent changes, which might be used as reference index for determination of age of skeletal muscle injury.

关 键 词：法医病理学 创伤和损伤 骨骼肌 多形核白细胞 单个核细胞 成纤维样细胞 大鼠 

分 类 号：DF795.1[医药卫生—法医学]